csgD Antibody

Code CSB-PA344759XA01ENV
Size US$299
Image
  • Western Blot
    Positive WB detected in: recombinant protein
    All lanes: csgD Antibody at 1:1000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 29 kDa
    Observed band size: 29 kDa
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Product Details

Uniprot No.
Target Names
csgD
Alternative Names
CsgBAC operon transcriptional regulatory protein csgD b1040 JW1023
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) csgD protein (1-216aa)
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

To generate the csgD polyclonal antibody, a recombinant Escherichia coli (strain K12) csgD protein (amino acids 1-216) is used as the immunogen, which is then administered to a rabbit to elicit an antibody response. The serum obtained from the rabbit contains polyclonal antibodies, which are subsequently purified using affinity chromatography. The proficiency of the csgD antibody in detecting the Escherichia coli (strain K12) csgD protein is assessed through ELISA and WB assays, confirming its suitability for experimental use.

The csgD protein in Escherichia coli (strain K12) is a transcriptional regulator crucial for orchestrating the synthesis and assembly of curli fibers, and extracellular amyloid structures with diverse functions. CsgD promotes the expression of genes involved in curli biosynthesis, export, and assembly. Additionally, CsgD-mediated adhesion to surfaces is pivotal for initial biofilm development and colonization of diverse environments, including host tissues.

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Target Background

Function
The master regulator for adhesive curli fimbriae expression; necessary for transcription of the csgBAC/ymdA operon. Plays a positive role in biofilm formation. May have the capability to respond to starvation and/or high cell density by activating csgBA transcription. Low-level constitutive expression confers an adherent curli fimbriae-expressing phenotype, up-regulates 10 genes and down-regulates 14 others.
Gene References into Functions
  1. Regulation of the master biofilm regulator, CsgD, was diverse, and the screen revealed several proteins and small RNAs (sRNA) that regulate csgD messenger RNA (mRNA) levels. Using previously published studies, we found minimal overlap between the genes affecting curli biogenesis and genes known to impact swimming or swarming motility, underlying the distinction between motile and sessile lifestyles. PMID: 29088115
  2. Promoter mutations in csgD that alter sigma factor utilization from RpoS to RpoD provide a unique mechanism for increasing biofilm formation, but the transition also disrupts normal rpoS-dependent CsgD regulation. PMID: 28406080
  3. RprA can directly reduce csgD mRNA levels and translation, and ce transcription of csgD. PMID: 22356413
  4. The authors propose that the cell motility for planktonic growth is repressed by CsgD, thereby promoting the switch to biofilm formation. PMID: 21421764
  5. Five different transcription factors interact to regulate the CsgD promoter. PMID: 20466769
  6. CsgD activation of glyA requires the participation of both known regulatory proteins, MetR and PurR PMID: 15644921
  7. role of the interplay OmpR and CpxR in affecting the transcription of the curli gene in response to variation of the medium osmolarity; CpxR represses csgD expression in high-salt-content medium, resulting in low curli production PMID: 15743952
  8. Our results indicate that CsgD can modulate cellulose biosynthesis through activation of the yoaD gene. In addition, the positive effect of CsgD on biofilm formation might be enhanced by repression of the fecR gene. PMID: 16513732

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Subcellular Location
Cell inner membrane; Peripheral membrane protein. Note=In experiments done with low-level constitutively expressed protein.
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