CKA2 Antibody

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Code	CSB-PA600863XA01DOA
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Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CKA2 Polyclonal antibody

Uniprot No.

Q08466

Target Names

CKA2

Alternative Names

CKA2 antibody; At3g50000 antibody; F3A4.80Casein kinase II subunit alpha-2 antibody; CK II antibody; EC 2.7.11.1 antibody; Casein kinase alpha 2 antibody; AtCKA2 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana CKA2 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha chain contains the catalytic site. The tetrameric holoenzyme CK2, composed of two alpha and two beta subunits, phosphorylates the transcription factor PIF1 after an exposure to light, resulting in a proteasome-dependent degradation of PIF1 and promotion of photomorphogenesis. CK2 phosphorylates translation initiation factors. May participate in the regulation of the initiation of translation. Acts as circadian clock component that maintains the correct period length through phosphorylation of CCA1. May act as an ectokinase that phosphorylates several extracellular proteins.

Gene References into Functions

CK2 activity increases the roots endogenous salicylic acid levels, indicating that CK2 activity is required for SA biosynthesis.CK2 is transcriptionally regulated by salicylic acid. PMID: 24547808
CK2 is required in the maintenance and control of genomic stability and chromatin structure in plants, and that this process affects several functions, including the DNA damage response and DNA repair.[CK2] PMID: 22487192
CK2 activity depletion affects auxin-dependent processes, and particularly auxin transport. PMID: 21435053
The effects of a cka1a2a3 triple mutant on the Arabidopsis (Arabidopsis thaliana) circadian clock, were examined. PMID: 21900482
alpha1 and alpha2 subunits of CK2 individually phosphorylated phytochrome interacting factors weakly in vitro. PMID: 21330376

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Subcellular Location

Nucleus. Nucleus, nucleolus.

Protein Families

Protein kinase superfamily, Ser/Thr protein kinase family, CK2 subfamily

Tissue Specificity

Seems to be present in all plant organs. But seems to be more expressed than CKA1.

Database Links

KEGG: ath:AT3G50000

STRING: 3702.AT3G50000.1

UniGene: At.268

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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CKA2 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background