Recombinant Arabidopsis thaliana Casein kinase II subunit alpha-2 (CKA2)

Code CSB-YP600863DOA
MSDS
Size Pls inquire
Source Yeast
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP600863DOA
MSDS
Size Pls inquire
Source E.coli
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP600863DOA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-BP600863DOA
MSDS
Size Pls inquire
Source Baculovirus
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-MP600863DOA
MSDS
Size Pls inquire
Source Mammalian cell
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Purity
>85% (SDS-PAGE)
Target Names
CKA2
Uniprot No.
Alternative Names
CKA2; At3g50000; F3A4.80Casein kinase II subunit alpha-2; CK II; EC 2.7.11.1; Casein kinase alpha 2; AtCKA2
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
32-403
Target Protein Sequence
LIPPLTCVN TGTVESDVTG IRFDRCLDTD SLAKISLSTV MSKARVYTDV NVIRPKDYWD YESLNVQWGE QDDYEVVRKV GRGKYSEVFE GINMNNNEKC IIKILKPVKK KKIRREIKIL QNLCGGPNIV KLLDVVRDQH SKTPSLIFEY VNSTDFKVLY PTLTDYDIRY YIYELLKALD FCHSQGIMHR DVKPHNVMID HELRKLRLID WGLAEFYHPG KEYNVRVASR YFKGPELLVD LQDYDYSLDM WSLGCMFAGM IFRKEPFFYG HDNQDQLVKI AKVLGTDELN AYLNKYQLEL DTQLEALVGR HSRKPWSKFI NADNRHLVSP EAIDYLDKLL RYDHQDRLTA KEAMAHPYFA QVRAAESSRM RTQ
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha chain contains the catalytic site. The tetrameric holoenzyme CK2, composed of two alpha and two beta subunits, phosphorylates the transcription factor PIF1 after an exposure to light, resulting in a proteasome-dependent degradation of PIF1 and promotion of photomorphogenesis. CK2 phosphorylates translation initiation factors. May participate in the regulation of the initiation of translation. Acts as circadian clock component that maintains the correct period length through phosphorylation of CCA1. May act as an ectokinase that phosphorylates several extracellular proteins.
Gene References into Functions
  1. CK2 activity increases the roots endogenous salicylic acid levels, indicating that CK2 activity is required for SA biosynthesis.CK2 is transcriptionally regulated by salicylic acid. PMID: 24547808
  2. CK2 is required in the maintenance and control of genomic stability and chromatin structure in plants, and that this process affects several functions, including the DNA damage response and DNA repair.[CK2] PMID: 22487192
  3. CK2 activity depletion affects auxin-dependent processes, and particularly auxin transport. PMID: 21435053
  4. The effects of a cka1a2a3 triple mutant on the Arabidopsis (Arabidopsis thaliana) circadian clock, were examined. PMID: 21900482
  5. alpha1 and alpha2 subunits of CK2 individually phosphorylated phytochrome interacting factors weakly in vitro. PMID: 21330376

Show More

Hide All

Subcellular Location
Nucleus. Nucleus, nucleolus.
Protein Families
Protein kinase superfamily, Ser/Thr protein kinase family, CK2 subfamily
Tissue Specificity
Seems to be present in all plant organs. But seems to be more expressed than CKA1.
Database Links

KEGG: ath:AT3G50000

STRING: 3702.AT3G50000.1

UniGene: At.268

icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1