CKB4 Antibody

Datasheet

Code	CSB-PA525692XA01DOA
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Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CKB4 Polyclonal antibody

Uniprot No.

O80507

Target Names

CKB4

Alternative Names

CKB4 antibody; At2g44680 antibody; F16B22.17Putative casein kinase II subunit beta-4 antibody; CK II beta-4 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana CKB4 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

Plays a complex role in regulating the basal catalytic activity of the alpha subunit. The tetrameric holoenzyme CK2, composed of two alpha and two beta subunits, phosphorylates the transcription factor PIF1 after an exposure to light, resulting in a proteasome-dependent degradation of PIF1 and promotion of photomorphogenesis. CK2 phosphorylates translation initiation factors. May participate in the regulation of the initiation of translation.

Gene References into Functions

elevated expression of CKB4 and increased protein kinase CK2 activity enhance the sensitivity of plants to Cd stress by affecting Cd and H2O2 accumulation, including the modulation of genes involved in Cd transport and H2O2 scavenging PMID: 27750098
Data show that each of four beta subunits of CK2 strongly stimulated phosphorylation of phytochrome interacting factors by alpha1 or alpha2. PMID: 21330376
This study establishes a new model demonstrating that two opposing and temperature-dependent activities (CCA1-CK2) are essential for clock temperature compensation in Arabidopsis. PMID: 21079791
CKB4, a CK2 regulatory subunit, is a component of the Arabidopsis circadian system. [CKB4] PMID: 16709199
Oscillator dysfunction is responsible for the period defect of CKB4-ox plants that leads to clock dissonance with the environment and reduced sensitivity to day length. PMID: 17662034

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Subcellular Location

Cytoplasm, cytosol.

Protein Families

Casein kinase 2 subunit beta family

Database Links

KEGG: ath:AT2G44680

STRING: 3702.AT2G44680.1

UniGene: At.12539

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Service

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Gene Synthesis Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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CKB4 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background