Recombinant Arabidopsis thaliana Putative casein kinase II subunit beta-4 (At2g44680)

Code CSB-YP525692DOA
MSDS
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Source Yeast
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Code CSB-EP525692DOA
MSDS
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Source E.coli
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Code CSB-EP525692DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP525692DOA
MSDS
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Source Baculovirus
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Code CSB-MP525692DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CKB4
Uniprot No.
Alternative Names
CKB4; At2g44680; F16B22.17Putative casein kinase II subunit beta-4; CK II beta-4
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-283
Target Protein Sequence
MYKDRSGGGI MGGGGSSRSE ILGGAIDRKR INDALDKHLK KSSPSTSRVF TSKDKDSVPS TSTAKSQLHS RSPDVESDTD SEGSDVSGSE GDDTSWISWF CNLRGNEFFC EVDEDYIQDD FNLCGLSGQV PYYDYALDLI LDVESSNGDM FTEEQHEMVE SAAEMLYGLI HVRYILTTKG MAAMMEKYKN YDFGRCPRVF CCGQSCLPVG QSDIPRSSTV KIYCPKCEDI YYPRSKYQGN IDGAYFGTTF PHLFLMAYGN MKPQKPAQNY VPKIFGFKVH NKQ
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Plays a complex role in regulating the basal catalytic activity of the alpha subunit. The tetrameric holoenzyme CK2, composed of two alpha and two beta subunits, phosphorylates the transcription factor PIF1 after an exposure to light, resulting in a proteasome-dependent degradation of PIF1 and promotion of photomorphogenesis. CK2 phosphorylates translation initiation factors. May participate in the regulation of the initiation of translation.
Gene References into Functions
  1. elevated expression of CKB4 and increased protein kinase CK2 activity enhance the sensitivity of plants to Cd stress by affecting Cd and H2O2 accumulation, including the modulation of genes involved in Cd transport and H2O2 scavenging PMID: 27750098
  2. Data show that each of four beta subunits of CK2 strongly stimulated phosphorylation of phytochrome interacting factors by alpha1 or alpha2. PMID: 21330376
  3. This study establishes a new model demonstrating that two opposing and temperature-dependent activities (CCA1-CK2) are essential for clock temperature compensation in Arabidopsis. PMID: 21079791
  4. CKB4, a CK2 regulatory subunit, is a component of the Arabidopsis circadian system. [CKB4] PMID: 16709199
  5. Oscillator dysfunction is responsible for the period defect of CKB4-ox plants that leads to clock dissonance with the environment and reduced sensitivity to day length. PMID: 17662034

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Subcellular Location
Cytoplasm, cytosol.
Protein Families
Casein kinase 2 subunit beta family
Database Links

KEGG: ath:AT2G44680

STRING: 3702.AT2G44680.1

UniGene: At.12539

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