Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) DCL3 Polyclonal antibody

Uniprot No.

Q9LXW7

Target Names

DCL3

Alternative Names

DCL3 antibody; At3g43920 antibody; T15B3.60Endoribonuclease Dicer homolog 3 antibody; EC 3.1.26.- antibody; Dicer-like protein 3 antibody; AtDCL3 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana DCL3 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Ribonuclease (RNase) III involved in RNA-mediated post-transcriptional gene silencing (PTGS). Involved in the processing of repeat-associated small interfering RNAs (ra-siRNAs, derived from heterochromatin and DNA repeats such as transposons) by cleaving small dsRNAs into 24 nucleotide ra-siRNAs. Plays a role in antiviral RNA silencing. Involved in the production of viral siRNAs derived from the cabbage leaf curl virus (CaLCuV) and tobacco rattle virus (TRV). Targeted by the viral silencing suppressor (VSR) protein 2b of the cucumber mosaic virus (CMV) that inactivates DCL3 function in RNA silencing. Acts redundantly with DICER-LIKE 1 (DCL1) to promote flowering via repression of FLOWERING LOCUS C (FLC). Does not seem to be involved in microRNAs (miRNAs) processing.

Gene References into Functions

Data demonstrate that different nutrient statuses of a host plant affect the post-translational regulation of the dicing activity of DCL3 and DCL4. Phosphate deficiency inhibited DCL3, and the activity of DCL3 was directly activated by inorganic phosphate. PMID: 28069892
This expression-dependent RdDM mechanism functions through RNAi degradation of transposable element mRNAs into small RNAs guided by the RNA-dependent RNA polymerase 6 (RDR6) protein and is therefore referred to as RDR6-RdDM.We identified a new mechanism of expression-dependent RdDM, which functions through DICER-LIKE3 (DCL3) but bypasses the requirement of both RNA polymerase IV and RDR6 (termed DCL3-RdDM). PMID: 27506905
The authors identified Pol IV and RDR2-dependent RNAs (P4R2 RNAs) that accumulate in dcl3 mutants and are diced into 24 nt RNAs by DCL3 in vitro. PMID: 26430765
CMA33/XCT modulates small RNA production through regulating the transcription of Dicer-Like Genes. PMID: 25770820
DCL3 preferentially cleaves short dsRNAs with 5' phosphorylated adenosine or uridine and a 1 nt 3' overhang. PMID: 24214956
The data suggest a mechanism whereby IBM1 regulates a series of protein-coding genes including DCL3 through the RdDM-dependent control of siRNA biogenesis. PMID: 22772985
DNA methylation establishment and maintenance defects present in sr45-1 mutants are enhanced in dcl3-1 mutant background, suggesting a synergistic cooperation between SR45 and DICER-LIKE3 (DCL3) in the RdDM pathway. PMID: 22274613
Data show that small interfering RNAs and larger RNA fragments corresponding miRNA target genes accumulate in wild-type plants but not in ago1, the RNA-dependent RNA polymerase mutants rdr2 and rdr6, or the Dicer-like mutants dcl1 and dcl3. PMID: 16798886
DICER-LIKE 1 and DICER-LIKE 3 are involved in the generation of small RNAs; mutants exhibit a delay in flowering. PMID: 17579240
DCL3 is required for reception of long-distance mRNA silencing. PMID: 17785412

Hide All

Subcellular Location

Nucleus, nucleolus.

Protein Families

Helicase family, Dicer subfamily

Database Links

KEGG: ath:AT3G43920

STRING: 3702.AT3G43920.2

UniGene: At.36217

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Code	CSB-PA868068XA01DOA
Size	Enquire
Have Questions?	Leave a Message or Start an on-line Chat

DCL3 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background