Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) DCP2 Polyclonal antibody

Uniprot No.

Q8GW31

Target Names

DCP2

Alternative Names

DCP2 antibody; TDT antibody; At5g13570 antibody; MSH12.3 antibody; mRNA-decapping enzyme subunit 2 antibody; AtDCP2 antibody; Protein DECAPPING 2 antibody; EC 3.6.1.62 antibody; M(7)GpppN-mRNA hydrolase DCP2 antibody; Protein TRIDENT antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana DCP2 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Catalytic component of the decapping complex necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Essential for postembryonic development, especially during the formation of the shoot apical meristem (SAM).

Gene References into Functions

DCP1 and DCP2 respond differently to environmental changes, indicating that P-bodies have diverse DCP1 and DCP2 proportions depending on environmental conditions. PMID: 25339350
We found that AS2 promotes DCP2 decapping activity, accelerates mRNA turnover rate, inhibits siRNA accumulation and functions as an endogenous suppressor of post-transcriptional gene silencing PMID: 26431425
A mutation in AtDCP2 (At5g13570) caused the co-suppression phenotype in the its1 mutant, which displayed increased transgene silencing. PMID: 22639932
DCP5 associates with DCP1 and DCP2 and is required for mRNA decapping in vivo. In spite of its association with DCP2, DCP5 has no effect on DCP2 decapping activity in vitro, suggesting that the effect on decapping in vivo is indirect. PMID: 19855049
AtDCP2 is essential for post-embryonic development and supplies new insights into the role of decapping-dependent mRNA turnover. PMID: 17485080
Mutational analysis of the putative RNA-binding domain of Dcp2 confirmed the functional significance of an 11-residue loop region and the conserved Box B. PMID: 18025047

Hide All

Subcellular Location

Cytoplasm, P-body. Note=The localization to P-body is VCS-dependent.

Protein Families

Nudix hydrolase family, DCP2 subfamily

Tissue Specificity

Expressed in seedlings, mostly in root tips, root hairs, and the vascular system. Also present in roots, leaves, stems, and flowers.

Database Links

KEGG: ath:AT5G13570

STRING: 3702.AT5G13570.2

UniGene: At.32052

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Code	CSB-PA965276XA01DOA
Size	Enquire
Have Questions?	Leave a Message or Start an on-line Chat

DCP2 Antibody

Product Details

Customer Reviews and Q&A

Target Background