DELTA-actitoxin-Aeq1a Antibody

Code CSB-PA351109ZA01ACN
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Product Details

Full Product Name
Rabbit anti-Actinia equina (Beadlet anemone) DELTA-actitoxin-Aeq1a Polyclonal antibody
Uniprot No.
Target Names
DELTA-actitoxin-Aeq1a
Alternative Names
DELTA-actitoxin-Aeq1a (DELTA-AITX-Aeq1a) (Equinatoxin II) (EqT II) (EqTII) (Equinatoxin-2)
Raised in
Rabbit
Species Reactivity
Actinia equina
Immunogen
Recombinant Actinia equina DELTA-actitoxin-Aeq1a protein
Immunogen Species
Actinia equina (Beadlet anemone)
Conjugate
Non-conjugate
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (12-14 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Pore-forming protein that forms cations-selective hydrophilic pores of around 1 nm and causes cardiac stimulation and hemolysis. Pore formation is a multi-step process that involves specific recognition of membrane sphingomyelin (but neither cholesterol nor phosphatidylcholine) using aromatic rich region and adjacent phosphocholine (POC) binding site, firm binding to the membrane (mainly driven by hydrophobic interactions) accompanied by the transfer of the N-terminal region to the lipid-water interface and finally pore formation after oligomerization of monomers. Cytolytic effects include red blood cells hemolysis, platelet aggregation and lysis, cytotoxic and cytostatic effects on fibroblasts. Lethality in mammals has been ascribed to severe vasospasm of coronary vessels, cardiac arrhythmia, and inotropic effects.
Subcellular Location
Secreted. Nematocyst. Target cell membrane. Note=Forms an alpha-helical membrane channel in the prey.
Protein Families
Actinoporin family, Sea anemone subfamily
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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