DHAR1 Antibody

Code CSB-PA863850XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) DHAR1 Polyclonal antibody
Uniprot No.
Target Names
DHAR1
Alternative Names
DHAR1 antibody; DHAR5 antibody; At1g19570 antibody; F14P1.9 antibody; F18O14.22 antibody; Glutathione S-transferase DHAR1 antibody; mitochondrial antibody; EC 2.5.1.18 antibody; Chloride intracellular channel homolog 1 antibody; CLIC homolog 1 antibody; Glutathione-dependent dehydroascorbate reductase 1 antibody; AtDHAR1 antibody; GSH-dependent dehydroascorbate reductase 1 antibody; mtDHAR antibody; EC 1.8.5.1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana DHAR1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Displays a dual function. As a soluble protein, exhibits glutathione-dependent thiol transferase and dehydroascorbate (DHA) reductase activities. Key component of the ascorbate recycling system. Involved in the redox homeostasis, especially in scavenging of ROS under oxidative stresses, subsequently to biotic or abiotic inducers. As a peripheral membrane protein, could also function as voltage-gated ion channel.
Gene References into Functions
  1. the response and functional importance of 3 potential routes for glutathione oxidation pathways mediated by glutathione S-transferases (GST), glutaredoxin-dependent peroxiredoxins (PRXII), and dehydroascorbate reductases (DHAR) in Arabidopsis during oxidative stress, are reported. PMID: 28782990
  2. A pivotal role of DHAR1 in the modulation of cellular redox states of ascorbate-glutathione cycle under photooxidative stress PMID: 27852156
  3. MDAR2 and DHAR5 are crucial for producing sufficient ascorbate to maintain the interaction between Piriformospora indica and Arabidopsis in a mutualistic state. [DHAR5] PMID: 19386380
  4. DHAR1 generates an ion conductance in heterologous systems through a CLIC-like channel activity. [AtDHAR1] PMID: 17267397
Subcellular Location
Mitochondrion. Cytoplasm, cytosol. Peroxisome. Membrane; Peripheral membrane protein. Note=Exists both as soluble protein and as membrane protein.
Protein Families
GST superfamily, DHAR family
Tissue Specificity
Expressed at least in roots and leaves.
Database Links

KEGG: ath:AT1G19570

STRING: 3702.AT1G19570.1

UniGene: At.24135

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