Recombinant Arabidopsis thaliana Glutathione S-transferase DHAR1, mitochondrial (DHAR1)

Code CSB-YP863850DOA
MSDS
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Source Yeast
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Code CSB-EP863850DOA
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Source E.coli
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Code CSB-EP863850DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP863850DOA
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Source Baculovirus
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Code CSB-MP863850DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
DHAR1
Uniprot No.
Alternative Names
DHAR1; DHAR5; At1g19570; F14P1.9; F18O14.22; Glutathione S-transferase DHAR1; mitochondrial; EC 2.5.1.18; Chloride intracellular channel homolog 1; CLIC homolog 1; Glutathione-dependent dehydroascorbate reductase 1; AtDHAR1; GSH-dependent dehydroascorbate reductase 1; mtDHAR; EC 1.8.5.1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-213
Target Protein Sequence
MALEICVKAA VGAPDHLGDC PFSQRALLTL EEKSLTYKIH LINLSDKPQW FLDISPQGKV PVLKIDDKWV TDSDVIVGIL EEKYPDPPLK TPAEFASVGS NIFGTFGTFL KSKDSNDGSE HALLVELEAL ENHLKSHDGP FIAGERVSAV DLSLAPKLYH LQVALGHFKS WSVPESFPHV HNYMKTLFSL DSFEKTKTEE KYVISGWAPK VNP
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Displays a dual function. As a soluble protein, exhibits glutathione-dependent thiol transferase and dehydroascorbate (DHA) reductase activities. Key component of the ascorbate recycling system. Involved in the redox homeostasis, especially in scavenging of ROS under oxidative stresses, subsequently to biotic or abiotic inducers. As a peripheral membrane protein, could also function as voltage-gated ion channel.
Gene References into Functions
  1. the response and functional importance of 3 potential routes for glutathione oxidation pathways mediated by glutathione S-transferases (GST), glutaredoxin-dependent peroxiredoxins (PRXII), and dehydroascorbate reductases (DHAR) in Arabidopsis during oxidative stress, are reported. PMID: 28782990
  2. A pivotal role of DHAR1 in the modulation of cellular redox states of ascorbate-glutathione cycle under photooxidative stress PMID: 27852156
  3. MDAR2 and DHAR5 are crucial for producing sufficient ascorbate to maintain the interaction between Piriformospora indica and Arabidopsis in a mutualistic state. [DHAR5] PMID: 19386380
  4. DHAR1 generates an ion conductance in heterologous systems through a CLIC-like channel activity. [AtDHAR1] PMID: 17267397
Subcellular Location
Mitochondrion. Cytoplasm, cytosol. Peroxisome. Membrane; Peripheral membrane protein. Note=Exists both as soluble protein and as membrane protein.
Protein Families
GST superfamily, DHAR family
Tissue Specificity
Expressed at least in roots and leaves.
Database Links

KEGG: ath:AT1G19570

STRING: 3702.AT1G19570.1

UniGene: At.24135

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