Product Details

Full Product Name

Rabbit anti-Mus musculus (Mouse) Dhx58 Polyclonal antibody

Uniprot No.

Q99J87

Target Names

Dhx58

Alternative Names

Dhx58 antibody; D11lgp2e antibody; Lgp2Probable ATP-dependent RNA helicase DHX58 antibody; EC 3.6.4.13 antibody; Probable ATP-dependent helicase LGP2 antibody; Protein D11Lgp2 antibody; RIG-I-like receptor 3 antibody; RLR-3 antibody; RIG-I-like receptor Lgp2 antibody; RLR antibody

Raised in

Rabbit

Species Reactivity

Mus musculus (Mouse)

Immunogen

Recombinant Mus musculus (Mouse) Dhx58 protein

Immunogen Species

Mus musculus (Mouse)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Acts as a regulator of DDX58/RIG-I and IFIH1/MDA5 mediated antiviral signaling. Cannot initiate antiviral signaling as it lacks the CARD domain required for activating MAVS/IPS1-dependent signaling events. Can have both negative and positive regulatory functions related to DDX58/RIG-I and IFIH1/MDA5 signaling and this role in regulating signaling may be complex and could probably depend on characteristics of the infecting virus or target cells, or both. Its inhibitory action on DDX58/RIG-I signaling may involve the following mechanisms: competition with DDX58/RIG-I for binding to the viral RNA, binding to DDX58/RIG-I and inhibiting its dimerization and interaction with MAVS/IPS1, competing with IKBKE in its binding to MAVS/IPS1 thereby inhibiting activation of interferon regulatory factor 3 (IRF3). Its positive regulatory role may involve unwinding or stripping nucleoproteins of viral RNA thereby facilitating their recognition by DDX58/RIG-I and IFIH1/MDA5. Involved in the innate immune response to various RNA viruses and some DNA viruses such as poxviruses, and also to the bacterial pathogen Listeria monocytogenes. Can bind both ssRNA and dsRNA, with a higher affinity for dsRNA. Shows a preference to 5'-triphosphorylated RNA, although it can recognize RNA lacking a 5'-triphosphate.

Gene References into Functions

PACT interacts with LGP2 and this interaction is enhanced by encephalomyocarditis virus (EMCV) infection. In vitro interaction analyses using purified recombinant proteins confirmed that the single-stranded Theiler's murine encephalitis virus genome enhanced the interaction between LGP2 and PACT. PMID: 29032202
This in vivo study reveals that LGP2 is a major downregulator of the influenza A virus-triggered detrimental inflammatory response. PMID: 24493823
Enhanced expression of LGP2 suppresses the IFN stimulated genes associated with cytotoxic stress by turning off the expression of IFNbeta PMID: 24434553
LGP2, a host protein induced during influenza A virus infection, downregulates the host antiviral IFN response PMID: 22837208
LGP2 promotes an essential prosurvival signal in response to antigen stimulation to confer CD8+ T cell-number expansion and effector functions against divergent RNA viruses, including West Nile virus and lymphocytic choriomeningitis virus. PMID: 22841161
findings demonstrate a regulatory role for LGP2 in the response to cytosolic DNA, an intracellular bacterial pathogen, and a DNA virus, and provide a plausible mechanistic hypothesis as the basis for this activity PMID: 21533147
Lgp2 acts as a negative feedback regulator of antiviral signaling by sequestering double-stranded RNA from retinoic acid-inducible gene-I (RIG-I). PMID: 16210631
Functional analysis of the human LGP2 ortholog. PMID: 17020950

Hide All

Subcellular Location

Cytoplasm.

Protein Families

Helicase family, RLR subfamily

Tissue Specificity

Highly expressed in mammary tissues. Expressed in liver and testis. Expressed at lower level in spleen, embryo, mammary gland and breast tumors.

Database Links

KEGG: mmu:80861

STRING: 10090.ENSMUSP00000017974

UniGene: Mm.271830

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Dhx58 Antibody

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