DIM Antibody

Code CSB-PA653690XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) DIM Polyclonal antibody
Uniprot No.
Target Names
DIM
Alternative Names
DIM antibody; CBB1 antibody; DWF1 antibody; EVE1 antibody; At3g19820 antibody; MPN9.6Delta(24)-sterol reductase antibody; EC 1.3.1.72 antibody; Cell elongation protein DIMINUTO antibody; Cell elongation protein Dwarf1 antibody; Protein CABBAGE1 antibody; Protein ENHANCED VERY-LOW-FLUENCE RESPONSE 1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana DIM protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Plays a critical role in the general process of plant cell elongation. Involved in the synthesis of campesterol, an early precursor of brassinolide. Required for the conversion of 24-methylenecholesterol to campesterol and for the conversion of isofucosterol to sitosterol. Necessary for both the isomerization and reduction of 24-methylenecholesterol. Regulates indirectly phytochrome-mediated light responses through the modulation of brassinosteroid biosynthesis.
Gene References into Functions
  1. Data indicate a role for DIM1 in secondary cell wall formation. PMID: 21947665
  2. The genomic location of the EVE1 gene in Arabidopsis thaliana corresponded to a bacterial artificial chromosome (BAC) F4C21 from chromosome IV at ~17cM which encoded a novel ubiquitin family protein (At4g03350), consisting of a single exon. PMID: 21624980
  3. Overproduction and repression of Dim/dwf1 resulted in contrasting phenotypes, with repressors mimicking the brd2 phenotype and overproducers having large stature with increased numbers of flowers and seeds. PMID: 15994910
  4. results provide direct proof that Ca2+/calmodulin-mediated signalling has a critical role in controlling the function of DWF1 [DWARF1] PMID: 16193053
Subcellular Location
Microsome membrane; Single-pass type II membrane protein; Cytoplasmic side.
Protein Families
DIMINUTO family
Database Links

KEGG: ath:AT3G19820

STRING: 3702.AT3G19820.1

UniGene: At.248

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