Recombinant Arabidopsis thaliana Delta (24)-sterol reductase (DIM), partial

Code CSB-YP653690DOA
MSDS
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Source Yeast
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Code CSB-EP653690DOA
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Source E.coli
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Code CSB-EP653690DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP653690DOA
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Source Baculovirus
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Code CSB-MP653690DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
DIM
Uniprot No.
Alternative Names
DIM; CBB1; DWF1; EVE1; At3g19820; MPN9.6Delta(24)-sterol reductase; EC 1.3.1.72; Cell elongation protein DIMINUTO; Cell elongation protein Dwarf1; Protein CABBAGE1; Protein ENHANCED VERY-LOW-FLUENCE RESPONSE 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Plays a critical role in the general process of plant cell elongation. Involved in the synthesis of campesterol, an early precursor of brassinolide. Required for the conversion of 24-methylenecholesterol to campesterol and for the conversion of isofucosterol to sitosterol. Necessary for both the isomerization and reduction of 24-methylenecholesterol. Regulates indirectly phytochrome-mediated light responses through the modulation of brassinosteroid biosynthesis.
Gene References into Functions
  1. Data indicate a role for DIM1 in secondary cell wall formation. PMID: 21947665
  2. The genomic location of the EVE1 gene in Arabidopsis thaliana corresponded to a bacterial artificial chromosome (BAC) F4C21 from chromosome IV at ~17cM which encoded a novel ubiquitin family protein (At4g03350), consisting of a single exon. PMID: 21624980
  3. Overproduction and repression of Dim/dwf1 resulted in contrasting phenotypes, with repressors mimicking the brd2 phenotype and overproducers having large stature with increased numbers of flowers and seeds. PMID: 15994910
  4. results provide direct proof that Ca2+/calmodulin-mediated signalling has a critical role in controlling the function of DWF1 [DWARF1] PMID: 16193053
Subcellular Location
Microsome membrane; Single-pass type II membrane protein; Cytoplasmic side.
Protein Families
DIMINUTO family
Database Links

KEGG: ath:AT3G19820

STRING: 3702.AT3G19820.1

UniGene: At.248

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