DIR1 Antibody

Code CSB-PA840926XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) DIR1 Polyclonal antibody
Uniprot No.
Target Names
DIR1
Alternative Names
DIR1 antibody; At5g48485 antibody; MJE7Putative lipid-transfer protein DIR1 antibody; Protein DEFECTIVE IN INDUCED RESISTANCE 1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana DIR1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Putative lipid transfer protein required for systemic acquired resistance (SAR) long distance signaling. May interact with a lipid-derived molecule to promote long distance signaling associated with SAR. Together with AZI1, required for glycerol-3-phosphate- (G3P) and azelaic acid- (AA) induced systemic acquired resistance (SAR). Component of plant systemic immunity involved in priming defenses in a AA-dependent manner, by modulating production and/or translocation of a mobile signal(s) during SAR. Is able to bind with high affinity monoacylated phospholipids, mainly lysophosphatidylcholines.
Gene References into Functions
  1. cell-to-cell movement of DIR1 through plasmodesmata is important during long-distance systemic acquired resistance signalling in Arabidopsis. PMID: 25296648
  2. The lipid transfer proteins DIR1 which is required for G3P- and azelaic acid-induced Systemic acquired resistance, is essential for G3P accumulation. PMID: 23602565
  3. DIR1 together with a glycerol-3-phosphate-derived compound are sufficient for the induction of systemic acquired resistance in wild type plants. PMID: 22067992
  4. DIR1 contains a signal sequence that directs it to the cell wall, and is upregulated during the basal resistance response to Pseudomonas syringae. PMID: 21896186
  5. implicated in systemic acquired resistance in planta, and crystallized PMID: 16820699
  6. The structure of DIR1 reveals a new type of lipid transfer protein. PMID: 18552128

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Subcellular Location
Secreted, extracellular space, apoplast. Endoplasmic reticulum. Cell junction, plasmodesma.
Protein Families
A9/FIL1 family
Database Links

KEGG: ath:AT5G48485

STRING: 3702.AT5G48485.1

UniGene: At.27209

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