Recombinant Arabidopsis thaliana Putative lipid-transfer protein DIR1 (DIR1)

Code CSB-YP840926DOA
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Source Yeast
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Code CSB-EP840926DOA
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Source E.coli
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Code CSB-EP840926DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP840926DOA
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Source Baculovirus
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Code CSB-MP840926DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
DIR1
Uniprot No.
Alternative Names
DIR1; At5g48485; MJE7Putative lipid-transfer protein DIR1; Protein DEFECTIVE IN INDUCED RESISTANCE 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
26-102
Target Protein Sequence
AIDLC GMSQDELNEC KPAVSKENPT SPSQPCCTAL QHADFACLCG YKNSPWLGSF GVDPELASAL PKQCGLANAP TC
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Putative lipid transfer protein required for systemic acquired resistance (SAR) long distance signaling. May interact with a lipid-derived molecule to promote long distance signaling associated with SAR. Together with AZI1, required for glycerol-3-phosphate- (G3P) and azelaic acid- (AA) induced systemic acquired resistance (SAR). Component of plant systemic immunity involved in priming defenses in a AA-dependent manner, by modulating production and/or translocation of a mobile signal(s) during SAR. Is able to bind with high affinity monoacylated phospholipids, mainly lysophosphatidylcholines.
Gene References into Functions
  1. cell-to-cell movement of DIR1 through plasmodesmata is important during long-distance systemic acquired resistance signalling in Arabidopsis. PMID: 25296648
  2. The lipid transfer proteins DIR1 which is required for G3P- and azelaic acid-induced Systemic acquired resistance, is essential for G3P accumulation. PMID: 23602565
  3. DIR1 together with a glycerol-3-phosphate-derived compound are sufficient for the induction of systemic acquired resistance in wild type plants. PMID: 22067992
  4. DIR1 contains a signal sequence that directs it to the cell wall, and is upregulated during the basal resistance response to Pseudomonas syringae. PMID: 21896186
  5. implicated in systemic acquired resistance in planta, and crystallized PMID: 16820699
  6. The structure of DIR1 reveals a new type of lipid transfer protein. PMID: 18552128

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Subcellular Location
Secreted, extracellular space, apoplast. Endoplasmic reticulum. Cell junction, plasmodesma.
Protein Families
A9/FIL1 family
Database Links

KEGG: ath:AT5G48485

STRING: 3702.AT5G48485.1

UniGene: At.27209

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