DMC1 Antibody

Code CSB-PA338672XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) DMC1 Polyclonal antibody
Uniprot No.
Target Names
DMC1
Alternative Names
DMC1 antibody; ISC2 antibody; YER179WMeiotic recombination protein DMC1 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) DMC1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Required for meiotic recombination, synaptonemal complex formation and cell cycle progression. Recruited to meiosis recombination hotspots by MEI5 and SAE3.
Gene References into Functions
  1. Dmc1's dominance in promoting strand exchange between homologs involves repression of Rad51's strand-exchange activity. PMID: 24367271
  2. Negative staining electron microscopy and iterative helical real-space reconstruction revealed that the ScDmc1-ssDNA nucleoprotein filament harbors 6.5 protomers per turn. PMID: 23769192
  3. study provides biochemical evidence that Rad51 acts as an accessory factor with Mei5-Sae3 for Dmc1-mediated joint molecule formation during meiosis; Rad51 is a multifunctional protein that catalyzes recombination directly in mitosis and indirectly, via Dmc1, during meiosis PMID: 22955832
  4. Dmc1-Mei5-Sae3 complex is integrated onto Rad51 ensembles and plays both catalytic and structural roles in interhomolog recombination during meiosis. PMID: 15620352
  5. Dmc1 forms rings with an external diameter of 18 nm and a central cavity of 4 nm PMID: 15835894
  6. data support the view that the active form of Dmc1 protein is a helical filament rather than a ring and that calcium plays a significant role in regulating meiotic recombination PMID: 16204247
  7. MND1/HOP2 facilitatea DMC1-dependent interhomolog crossover formation in meiosis. PMID: 16581767
  8. Deletion of DMC1 abolished DNA rereplication, but additional deletion of RAD17 restored the original phenotype PMID: 19116279

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Subcellular Location
Nucleus.
Protein Families
RecA family, DMC1 subfamily
Database Links

KEGG: sce:YER179W

STRING: 4932.YER179W

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