Recombinant Saccharomyces cerevisiae Meiotic recombination protein DMC1 (DMC1)

Code CSB-YP338672SVG
MSDS
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Source Yeast
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Code CSB-EP338672SVG
MSDS
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Source E.coli
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Code CSB-EP338672SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP338672SVG
MSDS
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Source Baculovirus
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Code CSB-MP338672SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
DMC1
Uniprot No.
Alternative Names
DMC1; ISC2; YER179WMeiotic recombination protein DMC1
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-334
Target Protein Sequence
MSVTGTEIDS DTAKNILSVD ELQNYGINAS DLQKLKSGGI YTVNTVLSTT RRHLCKIKGL SEVKVEKIKE AAGKIIQVGF IPATVQLDIR QRVYSLSTGS KQLDSILGGG IMTMSITEVF GEFRCGKTQM SHTLCVTTQL PREMGGGEGK VAYIDTEGTF RPERIKQIAE GYELDPESCL ANVSYARALN SEHQMELVEQ LGEELSSGDY RLIVVDSIMA NFRVDYCGRG ELSERQQKLN QHLFKLNRLA EEFNVAVFLT NQVQSDPGAS ALFASADGRK PIGGHVLAHA SATRILLRKG RGDERVAKLQ DSPDMPEKEC VYVIGEKGIT DSSD
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Required for meiotic recombination, synaptonemal complex formation and cell cycle progression. Recruited to meiosis recombination hotspots by MEI5 and SAE3.
Gene References into Functions
  1. Dmc1's dominance in promoting strand exchange between homologs involves repression of Rad51's strand-exchange activity. PMID: 24367271
  2. Negative staining electron microscopy and iterative helical real-space reconstruction revealed that the ScDmc1-ssDNA nucleoprotein filament harbors 6.5 protomers per turn. PMID: 23769192
  3. study provides biochemical evidence that Rad51 acts as an accessory factor with Mei5-Sae3 for Dmc1-mediated joint molecule formation during meiosis; Rad51 is a multifunctional protein that catalyzes recombination directly in mitosis and indirectly, via Dmc1, during meiosis PMID: 22955832
  4. Dmc1-Mei5-Sae3 complex is integrated onto Rad51 ensembles and plays both catalytic and structural roles in interhomolog recombination during meiosis. PMID: 15620352
  5. Dmc1 forms rings with an external diameter of 18 nm and a central cavity of 4 nm PMID: 15835894
  6. data support the view that the active form of Dmc1 protein is a helical filament rather than a ring and that calcium plays a significant role in regulating meiotic recombination PMID: 16204247
  7. MND1/HOP2 facilitatea DMC1-dependent interhomolog crossover formation in meiosis. PMID: 16581767
  8. Deletion of DMC1 abolished DNA rereplication, but additional deletion of RAD17 restored the original phenotype PMID: 19116279

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Subcellular Location
Nucleus.
Protein Families
RecA family, DMC1 subfamily
Database Links

KEGG: sce:YER179W

STRING: 4932.YER179W

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