Product Details

Full Product Name

Rabbit anti-Xenopus laevis (African clawed frog) dna2 Polyclonal antibody

Uniprot No.

Q8QHA5

Target Names

dna2

Alternative Names

dna2 antibody; dna2lDNA replication ATP-dependent helicase/nuclease DNA2 antibody; DNA replication ATP-dependent helicase-like homolog) [Includes: DNA replication nuclease DNA2 antibody; EC 3.1.-.-); DNA replication ATP-dependent helicase DNA2 antibody; EC 3.6.4.12)] antibody

Raised in

Rabbit

Species Reactivity

Xenopus laevis (African clawed frog)

Immunogen

Recombinant Xenopus laevis (African clawed frog) dna2 protein

Immunogen Species

Xenopus laevis (African clawed frog)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Key enzyme involved in DNA replication and DNA repair in nucleus and mitochondrion. Involved in Okazaki fragments processing by cleaving long flaps that escape fen1: flaps that are longer than 27 nucleotides are coated by replication protein A complex (RPA), leading to recruit dna2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double-strand break (DSB) repair by mediating the cleavage of 5'-ssDNA, while the 3'-ssDNA cleavage is prevented by the presence of RPA. Also involved in DNA replication checkpoint independently of Okazaki fragments processing. Possesses different enzymatic activities, such as single-stranded DNA (ssDNA)-dependent ATPase, 5'-3' helicase and endonuclease activities. While the ATPase and endonuclease activities are well-defined and play a key role in Okazaki fragments processing and DSB repair, the 5'-3' DNA helicase activity is subject to debate. According to various reports, the helicase activity is weak and its function remains largely unclear. Helicase activity may promote the motion of dna2 on the flap, helping the nuclease function.

Gene References into Functions

Data show that cleavage of free 5' DNA ends at clean double-strand breaks (DSBs) is mediated by DNA replication helicase/nuclease 2 (Dna2). PMID: 28981724
Dna2 co-localizes in foci with RPA and is found in a complex with replication fork components And-1 and Mcm10. Dna2 interacts with the DSB repair and checkpoint proteins Nbs1 and ATM. PMID: 20237432
Results provide strong evidence that xDNA2 is a major nuclease for the resection of DNA ends for homology-dependent DNA double-strand break repair in eukaryotes. PMID: 18820296

Subcellular Location

Nucleus. Mitochondrion.

Protein Families

DNA2/NAM7 helicase family

Database Links

KEGG: xla:378492

UniGene: Xl.17390

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dna2 Antibody

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