Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) DNA2 Polyclonal antibody

Uniprot No.

P38859

Target Names

DNA2

Alternative Names

DNA2 antibody; YHR164C antibody; DNA replication ATP-dependent helicase/nuclease DNA2 [Includes: DNA replication nuclease DNA2 antibody; EC 3.1.-.-); DNA replication ATP-dependent helicase DNA2 antibody; EC 3.6.4.12)] antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) DNA2 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Key enzyme involved in DNA replication and DNA repair. Involved in Okazaki fragments processing by cleaving long flaps that escape FEN1: flaps that are longer than 27 nucleotides are coated by replication protein A complex (RPA), leading to recruit DNA2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double-strand break (DSB) repair by mediating the cleavage of 5'-ssDNA. Possesses different enzymatic activities, such as single-stranded DNA (ssDNA)-dependent ATPase, 5'-3' helicase and endonuclease activities. While the ATPase and endonuclease activities are well-defined and play a key role in Okazaki fragments processing and DSB repair, the 5'-3' DNA helicase activity is atypical: it cannot load onto its tracking strand internally and has an absolute free 5'-end requirement. Helicase activity may promote the motion of DNA2 on the flap, helping the nuclease function.

Gene References into Functions

Replication intermediates that escape Dna2 activity are processed by Holliday junction resolvase Yen1. PMID: 27779184
role of the Dna2 translocase activity in DNA break end resection and in the imposition of the 5' strand specificity of end resection. PMID: 28336516
A model is proposed where Dna2 alone is responsible for cleaving of RPA-bound long DNA flaps. PMID: 26175049
Data show that Dna2 is required for normal telomeric CA-strand fill-in. PMID: 23963457
Nuclease activity of Saccharomyces cerevisiae Dna2 inhibits its potent DNA helicase activity. PMID: 23671118
N-terminal 45-kDa domain of Dna2 endonuclease/helicase targets the enzyme to secondary structure DNA PMID: 23344960
Saccharomyces cerevisiae Dna2, a nuclease-helicase that is essential for Okazaki fragment maturation, is employed specifically during S phase to stimulate Mec1 kinase and initiate the replication checkpoint PMID: 23355394
Results show that the yeast Dna2 cysteine cluster is indeed an Fe-S domain and present biochemical and genetic evidence that this domain plays a role not only in nuclease but also in helicase activity. PMID: 22684504
Cdk1-dependent phosphorylation of the resection nuclease Dna2 at Thr4, Ser17 and Ser237 stimulates its recruitment to double-strand breaks. PMID: 21841787
The DNA repair functions of Dna2 contribute to its essential function, and in the absence of this repair function, the cell cycle inhibition by the checkpoint leads to permanent arrest. PMID: 21508669
Dna2 exhibits a unique strand end-dependent helicase function PMID: 20929864
Dna2p and then FEN1 track to remove initiator RNA via long flap intermediates during Okazaki fragment maturation PMID: 15448135
DNA2 of Saccharomyces cerevisiae selectively unwinds triplet repeat DNA. PMID: 16085654
report here two novel activities of both the yeast and human Dna2 helicase/nuclease protein: single strand annealing and ATP-independent strand exchange on short duplexes PMID: 17032657
Replication protein A stimulates the 5'- to 3'-nuclease activity of Dna2 and inhibits the 3'- to 5'-exo/endonuclease activity of Dna2. PMID: 18593712
Dna2 and replication protein A coordinate their functions for efficient flap cleavage and preparation for flap endonuclease 1 PMID: 18799459

Hide All

Subcellular Location

Nucleus. Chromosome. Note=Recruited to double-strand. break (DSB) sites following phosphorylation at Ser-17 and Ser-237.

Protein Families

DNA2/NAM7 helicase family

Database Links

KEGG: sce:YHR164C

STRING: 4932.YHR164C

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DNA2 Antibody

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