DOF3 Antibody

Code CSB-PA169287XA01OFG
Size US$299
Image
  • Western Blot
    Positive WB detected in: recombinant protein
    All lanes:DOF3 Antibody at 1:1000
    Secondary
    Goat polyclonal to rabbit IgG at 1/20000 dilution
    Predicted band size: 22.5 kDa
    Observed band size: 27 kDa
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Product Details

Full Product Name
Rabbit anti-Oryza sativa subsp. japonica (Rice) DOF3 Polyclonal antibody
Uniprot No.
Target Names
DOF3
Alternative Names
Dof zinc finger protein 3 (OsDof3) (Prolamin box-binding factor) DOF3 RPBF Os02g0252400 LOC_Os02g15350 OSJNBa0009N02.17
Raised in
Rabbit
Species Reactivity
Oryza sativa subsp. japonica (Rice)
Immunogen
Recombinant Oryza sativa subsp. japonica (Rice) DOF3 protein (205-370aa)
Immunogen Species
Oryza sativa subsp. japonica (Rice)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Transcriptional activator that binds specifically to the DNA consensus core sequence 5'-AAAG-3' also known as prolamin box. Can activate the expression of genes encoding for the seed storage proteins glutelin, prolamin and globulin. Functions synergistically with RISBZ/BZIP58 to positively regulate quantitatively many seed storage proteins. Functions synergistically with RISBZ1/BZIP58 to positively regulate some metabolic enzymes, such as alanine aminotransferase and pyruvate phosphate dikinase, that are expressed in developing seeds. Functions synergistically with RISBZ1/BZIP58 to positively regulate genes that are key players in the development of aleurone layers. Functions synergistically with RISBZ1/BZIP58 to positively regulate the glutelin GLUD-1 gene in endosperm of developing seeds. Can activate the expression of the bifunctional lysine-degrading enzyme, lysine ketoglutarate reductase/saccharopine dehydrogenase (LKR/SDH), one of the key regulators determining free lysine content in plants. In germinating seeds, involved in the gibberellin-mediated activation of the alpha-amylase AMY1.1/AMY1A gene.
Subcellular Location
Nucleus.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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