DOF3.7 Antibody

Code CSB-PA673748XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) DOF3.7 Polyclonal antibody
Uniprot No.
Target Names
DOF3.7
Alternative Names
DOF3.7 antibody; BBFA antibody; DAG1 antibody; At3g61850 antibody; F21F14.20Dof zinc finger protein DOF3.7 antibody; AtDOF3.7 antibody; Dof affecting germination 1 antibody; RolB domain B factor a antibody; Transcription factor BBFa antibody; AtBBFa antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana DOF3.7 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcription factor specifically involved in the maternal control of seed germination. Regulates transcription by binding to a 5'-AA[AG]G-3' consensus core sequence. May ensure the inactivity of a component that would be activated to trigger germination as a consequence of red light perception.
Gene References into Functions
  1. GAI interacts with DAG1 in embryo development. PMID: 25064446
  2. DAG1 and the DELLA protein GAI cooperate in negatively regulating the AtGA3ox1 gene PMID: 24719470
  3. ELIP (EARLY LIGHT-INDUCED PROTEIN) genes of Arabidopsis are involved in seed germination and that they act downstream of the Dof protein DAG1, although they are not its direct regulatory targets. PMID: 21299564
Subcellular Location
Nucleus.
Tissue Specificity
Expressed in the phloem of the mother plant, including in roots, stem, leaves and flowers, but not present in the seed and embryo. In maturing siliques, found all through the funiculus connecting the placenta to the ovule, but not in the ovule.
Database Links

KEGG: ath:AT3G61850

UniGene: At.989

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