Recombinant Arabidopsis thaliana Dof zinc finger protein DOF3.7 (DOF3.7)

Code CSB-YP673748DOA
MSDS
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Source Yeast
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Code CSB-EP673748DOA
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Source E.coli
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Code CSB-EP673748DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP673748DOA
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Source Baculovirus
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Code CSB-MP673748DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
DOF3.7
Uniprot No.
Alternative Names
DOF3.7; BBFA; DAG1; At3g61850; F21F14.20Dof zinc finger protein DOF3.7; AtDOF3.7; Dof affecting germination 1; RolB domain B factor a; Transcription factor BBFa; AtBBFa
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-296
Target Protein Sequence
MDATKWTQGF QEMINVKPME QMISSTNNNT PQQQPTFIAT NTRPNATASN GGSGGNTNNT ATMETRKARP QEKVNCPRCN STNTKFCYYN NYSLTQPRYF CKGCRRYWTE GGSLRNVPVG GSSRKNKRSS TPLASPSNPK LPDLNPPILF SSQIPNKSNK DLNLLSFPVM QDHHHHALEL LRSNGVSSRG MNTFLPGQMM DSNSVLYSSL GFPTMPDYKQ SNNNLSFSID HHQGIGHNTI NSNQRAQDNN DDMNGASRVL FPFSDMKELS STTQEKSHGN NTYWNGMFSN TGGSSW
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcription factor specifically involved in the maternal control of seed germination. Regulates transcription by binding to a 5'-AA[AG]G-3' consensus core sequence. May ensure the inactivity of a component that would be activated to trigger germination as a consequence of red light perception.
Gene References into Functions
  1. GAI interacts with DAG1 in embryo development. PMID: 25064446
  2. DAG1 and the DELLA protein GAI cooperate in negatively regulating the AtGA3ox1 gene PMID: 24719470
  3. ELIP (EARLY LIGHT-INDUCED PROTEIN) genes of Arabidopsis are involved in seed germination and that they act downstream of the Dof protein DAG1, although they are not its direct regulatory targets. PMID: 21299564
Subcellular Location
Nucleus.
Tissue Specificity
Expressed in the phloem of the mother plant, including in roots, stem, leaves and flowers, but not present in the seed and embryo. In maturing siliques, found all through the funiculus connecting the placenta to the ovule, but not in the ovule.
Database Links

KEGG: ath:AT3G61850

UniGene: At.989

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