DOF4.7 Antibody

Code CSB-PA771142XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) DOF4.7 Polyclonal antibody
Uniprot No.
Target Names
DOF4.7
Alternative Names
DOF4.7 antibody; At4g38000 antibody; F20D10.120Dof zinc finger protein DOF4.7 antibody; AtDOF4.7 antibody; Protein DNA BINDING WITH ONE FINGER 4.7 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana DOF4.7 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcription factor that binds specifically to a 5'-AA[AG]G-3' consensus core sequence. Involved in the negative regulation of floral organ abscission by binding to the typical DOF 5'-AAAG-3' sequences in the promoter of ADPG2/PGAZAT, and by down-regulating its expression. ADPG2/PGAZAT is an abscission-related and cell wall hydrolyzing polygalacturonase. May act through the interaction with ZFP2, an abscission-related transcription factor.
Gene References into Functions
  1. AtDOF4.7 participates in the control of abscission as part of the transcription complex that directly regulates the expression of cell wall hydrolysis enzymes. [DOF4.7] PMID: 20466844
Subcellular Location
Nucleus.
Tissue Specificity
Highly expressed at the base of all organs of the flower, especially in the abscission zone (AZ) of petals, stamens and sepals. Expressed at low levels in sepals, filaments, stigmatic papillae, tips of young siliques, and at the base of pedicels and leaf
Database Links

KEGG: ath:AT4G38000

STRING: 3702.AT4G38000.1

UniGene: At.2738

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