Recombinant Arabidopsis thaliana Dof zinc finger protein DOF4.7 (DOF4.7)

Code CSB-YP771142DOA
MSDS
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Source Yeast
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Code CSB-EP771142DOA
MSDS
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Source E.coli
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Code CSB-EP771142DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP771142DOA
MSDS
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Source Baculovirus
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Code CSB-MP771142DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
DOF4.7
Uniprot No.
Alternative Names
DOF4.7; At4g38000; F20D10.120Dof zinc finger protein DOF4.7; AtDOF4.7; Protein DNA BINDING WITH ONE FINGER 4.7
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-246
Target Protein Sequence
MMTSSHQSNT TGFKPRRIKT TAKPPRQINN KEPSPATQPV LKCPRCDSVN TKFCYYNNYS LSQPRHYCKN CRRYWTRGGA LRNVPIGGST RNKNKPCSLQ VISSPPLFSN GTSSASRELV RNHPSTAMMM MSSGGFSGYM FPLDPNFNLA SSSIESLSSF NQDLHQKLQQ QRLVTSMFLQ DSLPVNEKTV MFQNVELIPP STVTTDWVFD RFATGGGATS GNHEDNDDGE GNLGNWFHNA NNNALL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcription factor that binds specifically to a 5'-AA[AG]G-3' consensus core sequence. Involved in the negative regulation of floral organ abscission by binding to the typical DOF 5'-AAAG-3' sequences in the promoter of ADPG2/PGAZAT, and by down-regulating its expression. ADPG2/PGAZAT is an abscission-related and cell wall hydrolyzing polygalacturonase. May act through the interaction with ZFP2, an abscission-related transcription factor.
Gene References into Functions
  1. AtDOF4.7 participates in the control of abscission as part of the transcription complex that directly regulates the expression of cell wall hydrolysis enzymes. [DOF4.7] PMID: 20466844
Subcellular Location
Nucleus.
Tissue Specificity
Highly expressed at the base of all organs of the flower, especially in the abscission zone (AZ) of petals, stamens and sepals. Expressed at low levels in sepals, filaments, stigmatic papillae, tips of young siliques, and at the base of pedicels and leaf
Database Links

KEGG: ath:AT4G38000

STRING: 3702.AT4G38000.1

UniGene: At.2738

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