DOF5.8 Antibody

Code CSB-PA887744XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) DOF5.8 Polyclonal antibody
Uniprot No.
Target Names
DOF5.8
Alternative Names
DOF5.8 antibody; At5g66940 antibody; K8A10.1Dof zinc finger protein DOF5.8 antibody; AtDOF5.8 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana DOF5.8 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcription factor that binds specifically to a 5'-AA[AG]G-3' consensus core sequence.
Gene References into Functions
  1. The overexpression of Dof5.8 modulates the auxin response and leads to impaired vein formation in A. thaliana.[Dof5.8] PMID: 25794540
  2. In this study, it is shown that MONOPTEROS (MP) directly activates the Dof5.8 promoter. Phenotypic analysis with the mp dof5.8 double mutants revealed that mutations within Dof5.8 enhanced the phenotype of a weak allele of mp. PMID: 25336688
  3. under abiotic stress conditions, ATDOF5.8 regulates the expression of ANAC069, and the activated ANAC069 binds to NAC recognition sequences or other motifs to regulate the expression of genes containing these motifs in their promoters. [ATDOF5.8] PMID: 25572919
Subcellular Location
Nucleus.
Database Links

KEGG: ath:AT5G66940

STRING: 3702.AT5G66940.1

UniGene: At.66743

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