Recombinant Arabidopsis thaliana Dof zinc finger protein DOF5.8 (DOF5.8)

Code CSB-YP887744DOA
MSDS
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Source Yeast
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Code CSB-EP887744DOA
MSDS
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Source E.coli
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Code CSB-EP887744DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP887744DOA
MSDS
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Source Baculovirus
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Code CSB-MP887744DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
DOF5.8
Uniprot No.
Alternative Names
DOF5.8; At5g66940; K8A10.1Dof zinc finger protein DOF5.8; AtDOF5.8
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-225
Target Protein Sequence
MPSEFSESRR VPKIPHGQGG SVAIPTDQQE QLSCPRCEST NTKFCYYNNY NFSQPRHFCK SCRRYWTHGG TLRDIPVGGV SRKSSKRSRT YSSAATTSVV GSRNFPLQAT PVLFPQSSSN GGITTAKGSA SSFYGGFSSL INYNAAVSRN GPGGGFNGPD AFGLGLGHGS YYEDVRYGQG ITVWPFSSGA TDAATTTSHI AQIPATWQFE GQESKVGFVS GDYVA
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcription factor that binds specifically to a 5'-AA[AG]G-3' consensus core sequence.
Gene References into Functions
  1. The overexpression of Dof5.8 modulates the auxin response and leads to impaired vein formation in A. thaliana.[Dof5.8] PMID: 25794540
  2. In this study, it is shown that MONOPTEROS (MP) directly activates the Dof5.8 promoter. Phenotypic analysis with the mp dof5.8 double mutants revealed that mutations within Dof5.8 enhanced the phenotype of a weak allele of mp. PMID: 25336688
  3. under abiotic stress conditions, ATDOF5.8 regulates the expression of ANAC069, and the activated ANAC069 binds to NAC recognition sequences or other motifs to regulate the expression of genes containing these motifs in their promoters. [ATDOF5.8] PMID: 25572919
Subcellular Location
Nucleus.
Database Links

KEGG: ath:AT5G66940

STRING: 3702.AT5G66940.1

UniGene: At.66743

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