dop-3 Antibody

Code CSB-PA740853XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans dop-3 Polyclonal antibody
Uniprot No.
Target Names
dop-3
Alternative Names
dop-3; T14E8.3; Dopamine receptor 3; Dopamine D2-like receptor dop-3
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans dop-3 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
G-protein coupled receptor which binds to the neurotransmitter dopamine with high affinity leading to the activation of an associated G-protein and downstream signaling pathways. Couples to G-proteins to inhibit adenylate cyclase (AC) activity and cAMP production. Antagonizes the D1-like dopamine receptor dop-1 to negatively regulate the rate of locomotion. In GABAergic, RIC, and SIA neurons, antagonizes the function of dop-1 to play a role in behavioral plasticity and regulate the decision-making process when conflicting alternatives are present. Antagonizes octopamine signaling in response to food by promoting the dopamine-mediated suppression of crh-1/CREB1 transcription factor activation in cholinergic SIA neurons. This is most likely in association with the G(o) alpha G-protein subunit goa-1. Promotes male mating behavior by antagonizing acetylcholine signaling to control the protrusion of copulatory spicules from the tail of males during hermaphrodite vulval location.
Gene References into Functions
  1. We demonstrate that dopamine, through DOP-3, negatively regulates NCA activity. PMID: 28968387
  2. dopaminergic signaling through DOP-3 normally acts to dampen ASH signaling and behavioral sensitivity to octanol PMID: 20209143
  3. Enhancement of odor avoidance learning requires dopamine signaling via dopamine receptor DOP-3. PMID: 21123582
  4. DOP-3 attenuates forskolin-stimulated cAMP formation in response to dopamine stimulation, whereas DOP-3nf does not. When DOP-3 was coexpressed with DOP-3nf, the ability to inhibit forskolin-stimulated cAMP formation was reduced. PMID: 16001968
Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
G-protein coupled receptor 1 family
Tissue Specificity
Expressed in the neurons of the head, ventral cord and tail with weak expression observed in body wall muscles and PVD neurons. In the ventral cord, expressed strongly in GABAergic neurons with weaker expression in cholinergic motor neurons. Expressed in
Database Links

KEGG: cel:CELE_T14E8.3

STRING: 6239.T14E8.3b

UniGene: Cel.355

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