Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) DPB2 Polyclonal antibody

Uniprot No.

P24482

Target Names

DPB2

Alternative Names

DPB2 antibody; YPR175W antibody; P9705.7DNA polymerase epsilon subunit B antibody; DNA polymerase II subunit 2 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) DPB2 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

As accessory component of the DNA polymerase epsilon complex participates in chromosomal DNA replication. It is required during synthesis of the leading and lagging DNA strands at the replication fork and binds at/or near replication origins and moves along DNA with the replication fork. It has 3'-5' proofreading exonuclease activity that correct errors arising during DNA replication. It is also involved in DNA synthesis during DNA repair.

Gene References into Functions

Dpb2, which participates in the early stages of DNA replication initiation, has an impact on the regulation of replication-related genes expression with possible implications for genomic stability. (Review) PMID: 28516230
dpb2-103 mutant cells progress through S phase faster than wild-type cells. Nrm1-dependent branch of the checkpoint is impaired in dpb2-103 cells. PMID: 28107343
The presence of the dpb2-100 mutant significantly decreases the interaction of Dpb2p with Psf1p and Psf3p. PMID: 25758782
Dpb2 integrates the leading-strand DNA polymerase into the eukaryotic replisome. PMID: 23499531
Authors propose that the essential function of Dpb2 is separate from the enzymatic activity of Pol epsilon in S. cerevisiae and that Dpb2 depends on a distinct domain at the very C-terminal end of the protein to physically interact with Pol2. PMID: 23034803
mechanism for interaction of Pol epsilon with DNA that might explain how the structure of the enzyme contributes to its intrinsic processivity PMID: 16369485
This DNA polymerase subunit other than those housing the DNA polymerase and 3' --> 5' exonuclease is essential in controlling the level of spontaneous mutagenesis and genetic stability in yeast cells. PMID: 18245343

Hide All

Subcellular Location

Cytoplasm. Nucleus.

Protein Families

DNA polymerase epsilon subunit B family

Database Links

KEGG: sce:YPR175W

STRING: 4932.YPR175W

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Code	CSB-PA332991XA01SVG
Size	Enquire
Have Questions?	Leave a Message or Start an on-line Chat

DPB2 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background