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Recombinant Saccharomyces cerevisiae DNA polymerase epsilon subunit B (DPB2), partial

Recombinant Saccharomyces cerevisiae DNA polymerase epsilon subunit B (DPB2), partial

Code	CSB-YP332991SVG
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP332991SVG
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP332991SVG-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP332991SVG
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP332991SVG
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

DPB2

Uniprot No.

P24482

Alternative Names

DPB2; YPR175W; P9705.7DNA polymerase epsilon subunit B; DNA polymerase II subunit 2

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

As accessory component of the DNA polymerase epsilon complex participates in chromosomal DNA replication. It is required during synthesis of the leading and lagging DNA strands at the replication fork and binds at/or near replication origins and moves along DNA with the replication fork. It has 3'-5' proofreading exonuclease activity that correct errors arising during DNA replication. It is also involved in DNA synthesis during DNA repair.

Gene References into Functions

Dpb2, which participates in the early stages of DNA replication initiation, has an impact on the regulation of replication-related genes expression with possible implications for genomic stability. (Review) PMID: 28516230
dpb2-103 mutant cells progress through S phase faster than wild-type cells. Nrm1-dependent branch of the checkpoint is impaired in dpb2-103 cells. PMID: 28107343
The presence of the dpb2-100 mutant significantly decreases the interaction of Dpb2p with Psf1p and Psf3p. PMID: 25758782
Dpb2 integrates the leading-strand DNA polymerase into the eukaryotic replisome. PMID: 23499531
Authors propose that the essential function of Dpb2 is separate from the enzymatic activity of Pol epsilon in S. cerevisiae and that Dpb2 depends on a distinct domain at the very C-terminal end of the protein to physically interact with Pol2. PMID: 23034803
mechanism for interaction of Pol epsilon with DNA that might explain how the structure of the enzyme contributes to its intrinsic processivity PMID: 16369485
This DNA polymerase subunit other than those housing the DNA polymerase and 3' --> 5' exonuclease is essential in controlling the level of spontaneous mutagenesis and genetic stability in yeast cells. PMID: 18245343