RRT2 Antibody

Code CSB-PA330864XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RRT2 Polyclonal antibody
Uniprot No.
Target Names
RRT2
Alternative Names
RRT2 antibody; DPH7 antibody; ERE1 antibody; YBR246W antibody; YBR1634Diphthine methyltransferase antibody; EC 3.1.1.97 antibody; Diphthamide biosynthesis protein 7 antibody; Endosomal recycling protein 1 antibody; Regulator of rDNA transcription protein 2 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RRT2 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Catalyzes the demethylation of diphthine methyl ester to form diphthine, an intermediate in diphthamide biosynthesis, a post-translational modification of histidine which occurs in translation elongation factor 2 (EFT1 and EFT2). Also plays a role in the regulation of the retromer complex and is required for the recycling from endosomes of plasma membrane proteins like CAN1 and MUP1. Identified in a screen for mutants with decreased levels of rDNA transcription.
Gene References into Functions
  1. Dph5-catalyzed reaction generates methylated diphthine, a previously overlooked intermediate, and Dph7 is a methylesterase that hydrolyzes methylated diphthine to produce diphthine and allows the Dph6-catalyzed amidation reaction to occur. PMID: 24739148
  2. It was shown that the YBR246W gene in yeast is required for the last step of diphthamide biosynthesis, as the deletion of YBR246W leads to the accumulation of diphthine, which is the enzymatic product of the second step of the biosynthesis. PMID: 22188241
  3. our data indicate that a complex containing the two novel proteins, Ere1 and Ere2, mediates cargo-specific recognition by the retromer pathway. [Ere1] PMID: 21880895
Subcellular Location
Cytoplasm. Endosome. Note=Recruited to endosomes in cells in which increased recycling of internalized plasma membrane proteins occurs.
Protein Families
DPH7 family
Database Links

KEGG: sce:YBR246W

STRING: 4932.YBR246W

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