Recombinant Saccharomyces cerevisiae Diphthamide biosynthesis protein RRT2 (RRT2)

Code CSB-YP330864SVG
MSDS
Size Pls inquire
Source Yeast
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP330864SVG
MSDS
Size Pls inquire
Source E.coli
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP330864SVG-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-BP330864SVG
MSDS
Size Pls inquire
Source Baculovirus
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-MP330864SVG
MSDS
Size Pls inquire
Source Mammalian cell
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Purity
>85% (SDS-PAGE)
Target Names
RRT2
Uniprot No.
Alternative Names
RRT2; DPH7; ERE1; YBR246W; YBR1634Diphthine methyltransferase; EC 3.1.1.97; Diphthamide biosynthesis protein 7; Endosomal recycling protein 1; Regulator of rDNA transcription protein 2
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-387
Target Protein Sequence
MDSIQESDVL NAVKTKLPPC CLRIFRNKII LVGTYDLDKS TGYRSGSLDV FTMDLKLLCS NNTYGAILDL KLSPFDDTLI CTAHSTGNIM LWRIRCTDKD DFQSNELDIH AIANLQLFEK DVLIASCHFS PLDCKKLLVT NTAGEAATID IRTLSVQFTA SAIAQAYSKL DKIDYEVQGA TEKVIHVESG QFLKPHELEC WTAEFGSLQP FQDVVFTGGD DSRIMAHDLR SKEFIWSNNR IHDAGVVSIK CSQPNFRNNK PTSIITGSYD DNIRSLDLRM MGESIFPGAN VPTVNKLACD LGGGVWRFVE SPIDQEQSHH NGSDRLLVCC MYNGAKVVTM NDNSDEYFQI QHYLKKGHDS MCYGGDWSNS LIATCSFYDN SLQTWIV
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
Catalyzes the demethylation of diphthine methyl ester to form diphthine, an intermediate in diphthamide biosynthesis, a post-translational modification of histidine which occurs in translation elongation factor 2 (EFT1 and EFT2). Also plays a role in the regulation of the retromer complex and is required for the recycling from endosomes of plasma membrane proteins like CAN1 and MUP1. Identified in a screen for mutants with decreased levels of rDNA transcription.
Gene References into Functions
  1. Dph5-catalyzed reaction generates methylated diphthine, a previously overlooked intermediate, and Dph7 is a methylesterase that hydrolyzes methylated diphthine to produce diphthine and allows the Dph6-catalyzed amidation reaction to occur. PMID: 24739148
  2. It was shown that the YBR246W gene in yeast is required for the last step of diphthamide biosynthesis, as the deletion of YBR246W leads to the accumulation of diphthine, which is the enzymatic product of the second step of the biosynthesis. PMID: 22188241
  3. our data indicate that a complex containing the two novel proteins, Ere1 and Ere2, mediates cargo-specific recognition by the retromer pathway. [Ere1] PMID: 21880895
Subcellular Location
Cytoplasm. Endosome. Note=Recruited to endosomes in cells in which increased recycling of internalized plasma membrane proteins occurs.
Protein Families
DPH7 family
Database Links

KEGG: sce:YBR246W

STRING: 4932.YBR246W

icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1