polA Antibody

Datasheet

Code	CSB-PA360555XA01ENV
Size	Enquire
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) polA Polyclonal antibody

Uniprot No.

P00582

Target Names

polA

Alternative Names

polA antibody; resA antibody; b3863 antibody; JW3835DNA polymerase I antibody; POL I antibody; EC 2.7.7.7 antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) polA protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function

In addition to polymerase activity, this DNA polymerase exhibits 3'-5' and 5'-3' exonuclease activity. It is able to utilize nicked circular duplex DNA as a template and can unwind the parental DNA strand from its template.

Gene References into Functions

Findings indicate an unrealized function of DNA polymerase I (Pol I), and a possible mechanism by which G4 structures can be resolved during DNA replication and/or repair. PMID: 28986969
Pol I catalyzes DNA synthesis past N6-dA-linked peptide cross-links and is likely to play an role in cellular bypass of large major groove DNA lesions. PMID: 21622737
analysis of complexes of DNA polymerase I (Klenow fragment) for base and sugar discrimination during nucleotide substrate selection PMID: 21362617
a new in vivo function for wild-type DNA polymerase I in chromosome termination at site(s) not yet identified is proposed PMID: 15752206
mechanism in which Arg668 forms a hydrogen bonding fork between the minor groove of the primer terminus and the ring oxygen of the deoxyribose moiety of the incoming dNTP to align the 3'-hydroxyl group with the alpha-phosphate of the dNTP PMID: 15823023
Glutamine677 is perhaps the first residue in the pol I family of enzymes that is shown to be essential for the functioning of both polymerase and proofreading activities. PMID: 15924429
mutant(Delta)polA is defective in base mismatch correction PMID: 16005896
analysis of amino acid substitutions that increase the fidelity of Escherichia coli DNA polymerase I PMID: 17301051
Data show that dnaX36 strains have an increased requirement for DNA polymerase I. PMID: 18156258

Hide All

Protein Families

DNA polymerase type-A family

Database Links

KEGG: ecj:JW3835

STRING: 316385.ECDH10B_4052

Promotions

Transmembrane Protein Questionnaire with Gifts

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Claudin 18.2, is a 'Dark Horse' in Anti-tumour Therapy? Decoding the Biological Complexity of Endometrial Cancer: Essential Insights for Researchers Information You Must Know about KCNN4 STAT3: a Key Transcriptional Regulator in Cancer MUC16 (CA125): a Promising Predictive Marker and Therapeutic Target in Cancer Two Types of Macrophages: M1 and M2 Macrophages NRG1: an Intriguing Therapeutic Target in Diseases Proteolysis Targeting Chimera (PROTAC): A CLDN6: A "New Favorite" in Targeted Cancer Therapy after CLDN 18.2! DDR1: a Novel Member of the RTKs Family, a Potential Target for Tumor Therapy! TPBG/5T4: the Oncofetal Protein, an Appealing Target for ADC or Vaccine Therapeutics in Tumors! CD93, A Rising Star in Anti-Tumor Therapy Dual Role of TNFRSF14 in Immunology Cell Differentiation Determines the Fate of Cells CLDN4: A Potential Prognostic Indicator and Therapeutic Target in Tumors！ What Are the Best Methods for Protein Purification? A Practical Guide A Comprehensive Overview of Ovarian Cancer Microglial Markers: Insights into Microglial Activation and Function Costimulatory/Coinhibitory: Is B7-H3 An Enemy or A Friend? Glial Fibrillary Acidic Protein - Astrocyte Specific Marker CEACAM8: a Member of CEA Family, Emerging as a Vital Player in Gynaecological or Gastrointestinal Cancer Research! The Metabolic Phenotype of Tumor-Associated Macrophages in the Tumor Microenvironment CEACAM5: A Promising Member of CEACAM Family, A Potential Target for ADC Drug Research! LPS/TLR4 Signal Transduction Pathway VSIG4: a Prominent B7 Family Costimulatory Molecule or Complement Receptor in Cancers and Inflammatory Diseases! How do Th1 and Th2 Cells Differentiate? What A Paradoxical TNF Signaling Pathway-Based on Different Receptors What Essential Biological Knowledge is Needed for Hepatocellular Carcinoma Research? SIGIRR: A Novel Member of the TIR Superfamily Acts as the Anti-Inflammatory 'Brake' to Control Immune Response in Various Diseases! CD70 (CD27L/TNFSF7): a TNF Receptor Member, a “Star” Target for Clinical Research in Systemic Lupus Erythematosus (SLE), Arthritis, and Tumors! CRTAM: Class I Restricted T cell-associated Molecule, an Intriguing New Member of the Ig-SF Family! Does TNF Superfamily Inhibit Cancer or Promote Its Development? GLP-1R: An Energy Metabolism Regulator of GPCR Family, an Effective Drug Target for Type 2 Diabetes Therapy! Mucin-17/MUC17: An Unexpected Target for Tumor Immune Therapy, Notably Gastric Cancer! Myosin Regulatory Light Chains (MYL12A and MYL12B): the New Functional Ligands for CD69, Potential Targets for Cardiac Disease or Tumors Research? Do You Really Know Neurons and Glia Cells in Nerve System? Itaconate is a negative regulator of inflammation The Overview of Growth Factor The Overview of Autophagy Crosstalk Between Autophagy and Apoptosis The overview of Wnt signaling pathway Chemokine Receptor CCR6: the Specific Receptor for CCL20, a Potential Target for Drug Research in Inflammatory Diseases and Gastrointestinal Cancer! CXCR4: An Attractive Target of GPCR family Brings Promising New Drugs for Cancer Therapy！ CLDN3: Another Tight Junction Protein or New Drug Target, Interest in CLDN Family is Growing Fast! Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

Join us with

Subscribe newsletter

Leave a message

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

polA Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background