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Recombinant Escherichia coli DNA polymerase I (polA), partial

Recombinant Escherichia coli DNA polymerase I (polA), partial

Code	CSB-YP360555ENV
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP360555ENV
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP360555ENV-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP360555ENV
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP360555ENV
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

polA

Uniprot No.

P00582

Alternative Names

polA; resA; b3863; JW3835DNA polymerase I; POL I; EC 2.7.7.7

Species

Escherichia coli (strain K12)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

In addition to polymerase activity, this DNA polymerase exhibits 3'-5' and 5'-3' exonuclease activity. It is able to utilize nicked circular duplex DNA as a template and can unwind the parental DNA strand from its template.

Gene References into Functions

Findings indicate an unrealized function of DNA polymerase I (Pol I), and a possible mechanism by which G4 structures can be resolved during DNA replication and/or repair. PMID: 28986969
Pol I catalyzes DNA synthesis past N6-dA-linked peptide cross-links and is likely to play an role in cellular bypass of large major groove DNA lesions. PMID: 21622737
analysis of complexes of DNA polymerase I (Klenow fragment) for base and sugar discrimination during nucleotide substrate selection PMID: 21362617
a new in vivo function for wild-type DNA polymerase I in chromosome termination at site(s) not yet identified is proposed PMID: 15752206
mechanism in which Arg668 forms a hydrogen bonding fork between the minor groove of the primer terminus and the ring oxygen of the deoxyribose moiety of the incoming dNTP to align the 3'-hydroxyl group with the alpha-phosphate of the dNTP PMID: 15823023
Glutamine677 is perhaps the first residue in the pol I family of enzymes that is shown to be essential for the functioning of both polymerase and proofreading activities. PMID: 15924429
mutant(Delta)polA is defective in base mismatch correction PMID: 16005896
analysis of amino acid substitutions that increase the fidelity of Escherichia coli DNA polymerase I PMID: 17301051
Data show that dnaX36 strains have an increased requirement for DNA polymerase I. PMID: 18156258