DREB1B Antibody

Code CSB-PA311307XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) DREB1B Polyclonal antibody
Uniprot No.
Target Names
DREB1B
Alternative Names
DREB1B antibody; CBF1 antibody; ERF029 antibody; At4g25490 antibody; M7J2.140Dehydration-responsive element-binding protein 1B antibody; Protein DREB1B antibody; C-repeat/dehydration-responsive element-binding factor 1 antibody; C-repeat-binding factor 1 antibody; CRT/DRE-binding factor 1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana DREB1B protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcriptional activator that binds specifically to the DNA sequence 5'-[AG]CCGAC-3'. Binding to the C-repeat/DRE element mediates cold-inducible transcription. CBF/DREB1 factors play a key role in freezing tolerance and cold acclimation.
Gene References into Functions
  1. Arabidopsis CBF1 gene provided protection and conferred cold tolerance to transgenic tomato without any phenotypic variation. PMID: 21287175
  2. The PKKPAGR sequence of CBF1 is essential for its transcriptional activity. Deletion of the sequence or mutations within it greatly impaired the ability of CBF1 to induce expression of its target genes. PMID: 19948259
  3. plays an important role in protection of PSII and PSI during the chilling stress under low irradiance PMID: 20022137
  4. SOC1 Directly Represses the Expression of CBF1 Genes. PMID: 19825833
  5. CBF1 domains bind to a target DNA motif with a universal CG step core recognition and different flanking bases preference PMID: 19878300
  6. Ectopic expression of AtCBF1 was sufficient to significantly increase the freezing tolerance of non-acclimated leaves and stems relative to wild-type plants. PMID: 17080948
  7. Transgenic expression of CBF1 in 2 Solanum species suggested an endogenous CBFpathway is involved in many of the alterations associated with cold acclimation. PMID: 18182016
  8. Data show that transgenic plants that constitutively express CBF1 accumulate less bioactive GA and as a consequence exhibit dwarfism and late flowering, and that DELLAs are components of the CBF1-mediated cold stress response. PMID: 18757556

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Subcellular Location
Nucleus.
Protein Families
AP2/ERF transcription factor family, ERF subfamily
Database Links

KEGG: ath:AT4G25490

STRING: 3702.AT4G25490.1

UniGene: At.232

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