Recombinant Arabidopsis thaliana Dehydration-responsive element-binding protein 1B (DREB1B)

Code CSB-YP311307DOA
MSDS
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Source Yeast
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Code CSB-EP311307DOA
MSDS
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Source E.coli
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Code CSB-EP311307DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP311307DOA
MSDS
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Source Baculovirus
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Code CSB-MP311307DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
DREB1B
Uniprot No.
Alternative Names
DREB1B; CBF1; ERF029; At4g25490; M7J2.140Dehydration-responsive element-binding protein 1B; Protein DREB1B; C-repeat/dehydration-responsive element-binding factor 1; C-repeat-binding factor 1; CRT/DRE-binding factor 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-213
Target Protein Sequence
MNSFSAFSEM FGSDYEPQGG DYCPTLATSC PKKPAGRKKF RETRHPIYRG VRQRNSGKWV SEVREPNKKT RIWLGTFQTA EMAARAHDVA ALALRGRSAC LNFADSAWRL RIPESTCAKD IQKAAAEAAL AFQDETCDTT TTNHGLDMEE TMVEAIYTPE QSEGAFYMDE ETMFGMPTLL DNMAEGMLLP PPSVQWNHNY DGEGDGDVSL WSY
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcriptional activator that binds specifically to the DNA sequence 5'-[AG]CCGAC-3'. Binding to the C-repeat/DRE element mediates cold-inducible transcription. CBF/DREB1 factors play a key role in freezing tolerance and cold acclimation.
Gene References into Functions
  1. Arabidopsis CBF1 gene provided protection and conferred cold tolerance to transgenic tomato without any phenotypic variation. PMID: 21287175
  2. The PKKPAGR sequence of CBF1 is essential for its transcriptional activity. Deletion of the sequence or mutations within it greatly impaired the ability of CBF1 to induce expression of its target genes. PMID: 19948259
  3. plays an important role in protection of PSII and PSI during the chilling stress under low irradiance PMID: 20022137
  4. SOC1 Directly Represses the Expression of CBF1 Genes. PMID: 19825833
  5. CBF1 domains bind to a target DNA motif with a universal CG step core recognition and different flanking bases preference PMID: 19878300
  6. Ectopic expression of AtCBF1 was sufficient to significantly increase the freezing tolerance of non-acclimated leaves and stems relative to wild-type plants. PMID: 17080948
  7. Transgenic expression of CBF1 in 2 Solanum species suggested an endogenous CBFpathway is involved in many of the alterations associated with cold acclimation. PMID: 18182016
  8. Data show that transgenic plants that constitutively express CBF1 accumulate less bioactive GA and as a consequence exhibit dwarfism and late flowering, and that DELLAs are components of the CBF1-mediated cold stress response. PMID: 18757556

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Subcellular Location
Nucleus.
Protein Families
AP2/ERF transcription factor family, ERF subfamily
Database Links

KEGG: ath:AT4G25490

STRING: 3702.AT4G25490.1

UniGene: At.232

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