Mkp3 Antibody

Code CSB-PA893338XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) Mkp3 Polyclonal antibody
Uniprot No.
Target Names
Mkp3
Alternative Names
Mkp3 antibody; CG14080Dual specificity protein phosphatase Mpk3 antibody; EC 3.1.3.16 antibody; EC 3.1.3.48 antibody; Drosophila MKP3 antibody; DMKP3 antibody; Mitogen-activated protein kinase phosphatase 3 antibody; MAP kinase phosphatase 3 antibody; MKP-3 antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) Mkp3 protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Negatively regulates the activity of members of the MAP kinase family in response to changes in the cellular environment. Has a specificity for the ERK family. Acts as negative regulator in a variety of developmental processes including cell differentiation and proliferation controlled by the Ras/ERK pathway. Suppresses the photoreceptor cell differentiation and wing vein formation. Required for proper oogenesis and early embryogenesis. Functions autonomously in a subset of photoreceptor progenitor cells in eye imaginal disks. Appears also to be required in surrounding non-neuronal cells for ommatidial patterning and photoreceptor differentiation. Plays a role in the maintenance of epithelial integrity during tracheal development.
Gene References into Functions
  1. We show that Tay bridge antagonizes EGFR signalling in the Drosophila melanogaster wing disc and other tissues, and that the protein interacts with both Erk and Mkp3 PMID: 24348264
  2. MKP-3 is critically involved in cell differentiation, proliferation, and gene expression by suppressing the Ras/ERK pathway, specifically binding to ERK via the N-terminal ERK-binding domain of MKP-3. PMID: 14701731
  3. Drosophila ERK specifically regulates DMKP-3 in terms of its enzyme activity and protein stability esssential for regulation. PMID: 15033693
  4. Study suggests that Mkp3 constitutes a conserved negative feedback loop on the activity of the Ras/ERK signaling pathway. PMID: 15704110
Subcellular Location
Cytoplasm.
Protein Families
Protein-tyrosine phosphatase family, Non-receptor class dual specificity subfamily
Tissue Specificity
Ubiquitous expression in eye and wing imaginal disks. Enriched in ovary.
Database Links

KEGG: dme:Dmel_CG14080

STRING: 7227.FBpp0303122

UniGene: Dm.3967

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