Recombinant Drosophila melanogaster Dual specificity protein phosphatase Mpk3 (Mkp3)

Code CSB-YP893338DLU
MSDS
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Source Yeast
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Code CSB-EP893338DLU
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Source E.coli
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Code CSB-EP893338DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP893338DLU
MSDS
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Source Baculovirus
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Code CSB-MP893338DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Mkp3
Uniprot No.
Alternative Names
Mkp3; CG14080Dual specificity protein phosphatase Mpk3; EC 3.1.3.16; EC 3.1.3.48; Drosophila MKP3; DMKP3; Mitogen-activated protein kinase phosphatase 3; MAP kinase phosphatase 3; MKP-3
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-411
Target Protein Sequence
MPETEHETCS KEWLQSQLRS LDSKDLILLD CRGSHEYSES HIRGAVNLCI PSIVLRRLAV GKIDLASTIK SPELKQRIQS GYKLCWFILY NGEGVPGQNQ EIAGAGSLAV AMDSIISILH RRLKQDGCRV VALQDGFNNF RQAFPEWCED DNQTHSKEIE SSRNVQTDQL MGLRSLRIST TQSDSACSSS AESSDCESSS HHHHHHSHHN YNEAPVEIIP GLLFLGNATH SCDSEALKKY NIKYVLNVTP DLPNKFKESG DIKYLQIPIT DHYSQDLAIH FPDAIQFIEE ARSASSVVLV HCLAGVSRSV TVTLAYLMHT RGLSLNDAFA MVRDRKPDVS PNFHFMQQLL SFESQLRLRP GSRFSCSCIA PDCNCMQTTG FMATHLANAT GVSPDSGIEF DRWTPSDTGL K
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Negatively regulates the activity of members of the MAP kinase family in response to changes in the cellular environment. Has a specificity for the ERK family. Acts as negative regulator in a variety of developmental processes including cell differentiation and proliferation controlled by the Ras/ERK pathway. Suppresses the photoreceptor cell differentiation and wing vein formation. Required for proper oogenesis and early embryogenesis. Functions autonomously in a subset of photoreceptor progenitor cells in eye imaginal disks. Appears also to be required in surrounding non-neuronal cells for ommatidial patterning and photoreceptor differentiation. Plays a role in the maintenance of epithelial integrity during tracheal development.
Gene References into Functions
  1. We show that Tay bridge antagonizes EGFR signalling in the Drosophila melanogaster wing disc and other tissues, and that the protein interacts with both Erk and Mkp3 PMID: 24348264
  2. MKP-3 is critically involved in cell differentiation, proliferation, and gene expression by suppressing the Ras/ERK pathway, specifically binding to ERK via the N-terminal ERK-binding domain of MKP-3. PMID: 14701731
  3. Drosophila ERK specifically regulates DMKP-3 in terms of its enzyme activity and protein stability esssential for regulation. PMID: 15033693
  4. Study suggests that Mkp3 constitutes a conserved negative feedback loop on the activity of the Ras/ERK signaling pathway. PMID: 15704110
Subcellular Location
Cytoplasm.
Protein Families
Protein-tyrosine phosphatase family, Non-receptor class dual specificity subfamily
Tissue Specificity
Ubiquitous expression in eye and wing imaginal disks. Enriched in ovary.
Database Links

KEGG: dme:Dmel_CG14080

STRING: 7227.FBpp0303122

UniGene: Dm.3967

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