EIN2 Antibody

Rare Species
Code CSB-PA874354XA01DOA
Size US$299
Image
  • Western Blot
    Positive WB detected in Recombinant protein
    All lanes: EIN2 antibody at 1:2000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 35 kDa
    Observed band size: 35 kDa
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Product Details

Uniprot No.
Target Names
EIN2
Alternative Names
Ethylene-insensitive protein 2 (AtEIN2) (EIN-2) (Cytokinin-resistant protein AtCKR1) (Protein ORESARA 3) [Cleaved into: EIN2-CEND (EIN2C)] EIN2 CKR1 ORE3 At5g03280 F12E4.10 MOK16.19
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana EIN2 protein (747-927aa)
Immunogen Species
Arabidopsis thaliana
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Protein G
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Central factor in signaling pathways regulated by ethylene (ET), and involved in various processes including development, plant defense, senescence, nucleotide sugar flux, and tropisms. Necessary for ethylene-mediated gene regulation, and for the induction of some genes by ozone. Acts downstream of ET receptors, and upstream of ethylene regulated transcription factors. Required for cytokinin-mediated processes. Seems to be implicated in cross-talk between ET, jasmonate and other pathways. Probably not involved in iron uptake. Has a short half-life and undergoes rapid proteasome-mediated turnover in the absence of ethylene. Required for ethylene-induced EIN3 stabilization via proteasomal degradation of EBF1/EBF2 proteins. Regulates the leaf senescence induced by methyl jasmonate, ethylene and abscisic acid (Ref.17, PubMed:15010611). Required during salt stress to confer resistance.; Trafficking signal inducing ethylene response. The nuclear localization is both necessary and sufficient to activate EIN3-mediated transcription and ethylene responses. Necessary and sufficient for 3'-UTR-mediated translational repression of EBF1 and EBF2 mRNAs. Ethylene induces EIN2-CEND to associate with 3' UTRs in cytoplasmic foci and target EBF1/2 mRNAs to cytoplasmic processing-body (P-body). MPK6 regulates the cleavage and nuclear translocation of EIN2-CEND under methyl jasmonate treatment. Required for EIN3 accumulation.
Gene References into Functions
  1. ENAP1 may potentially mediate ethylene-induced histone acetylation via its interactions with EIN2 C terminus. PMID: 27694846
  2. In the presence of ethylene, EIN2 interacts with ENAP1, elevating the levels of H3K14Ac and H3K23Ac, promoting more EIN3 binding to the targets shared with ENAP1 and resulting in a rapid transcriptional regulation. PMID: 28874528
  3. These results indicate that root responses to 6-pentyl-2H-pyran-2-one involve components of auxin transport and signaling and the ethylene-response modulator EIN2. PMID: 26568541
  4. role in regulating organ growth by restricting cell expansion PMID: 26039475
  5. Study reports another mechanism of EIN2-mediated ethylene signaling, whereby EIN2 imposes the translational repression of EBF1 and EBF2 mRNA and find that the EBF1/2 3' UTRs mediate EIN2-directed translational repression and identify multiple poly-uridylates (PolyU) motifs as functional cis elements of 3' UTRs. PMID: 26496607
  6. Characterization of one of the targets of the translation regulatory machinery, the ethylene signaling component EBF2, indicates that the signaling molecule EIN2 and the nonsense-mediated decay proteins UPFs play a central role in this ethylene-induced translational response. PMID: 26496608
  7. The data provide insight into an EIN2-mediated senescence signalling pathway that coordinates global gene expression during leaf senescence via a gene regulatory network. PMID: 24659488
  8. the ore1 loss-of-function mutants were epistatic to nes1, suggesting the dominant role of ORE1 and the antagonistic role of NES1 during nitric oxide-induced cotyledon senescence in Arabidopsis. PMID: 24336389
  9. EIN2 may be cleaved at alternative positions PMID: 23656878
  10. Interactions between ECIP1 and EIN2 or ethylene receptors regulate ethylene response and stress response. PMID: 21631530
  11. Data indicate that the expression and phosphorylation of ERF110 were regulated by ethylene via both Ethylene-Insensitive2-dependent and -independent pathways, which constitute a dual-and-opposing mechanism for ethylene in the regulation of bolting. PMID: 23188807
  12. study demonstrated that EIN2 harboring a functional nuclear localization signal is subjected to a cleavage event, followed by transporting its carboxyl end (CEND) into the nucleus, where CEND acts to stabilize EIN3 and activate ethylene responses PMID: 23070300
  13. reducing jasmonic acid levels, either mutationally or chemically, caused ein2 ethylene-insensitive mutants to become ethylene responsive. This effect was not seen with the ethylene-insensitive etr1-1 mutants that affect receptor function. PMID: 23219920
  14. involved in nitric oxide signaling in the regulation of leaf senescence PMID: 22765302
  15. These findings uncover a mechanism of subcellular communication whereby ethylene stimulates phosphorylation-dependent cleavage and nuclear movement of the EIN2-C' peptide, linking hormone perception and signaling components in the ER with nuclear-localized transcriptional regulators. PMID: 22936567
  16. These data suggest a novel model on the integration of EIN2 in the ethylene signaling pathway. PMID: 21242723
  17. AtMYB44 regulates induced expression of EIN2 in HrpN(Ea)-treated Arabidopsis plants. AtMYB44 protein localizes to nuclei and binds the EIN2 promoter; HrpN(Ea) treatment promotes AtMYB44 production, binding activity, and transcription of AtMYB44 and EIN2. PMID: 21117868
  18. Data show that protein phosphorylation is a key mechanism to control the interaction of EIN2 and the receptors. PMID: 20591837
  19. The etr1-3 and ein2-1 mutants exhibited less reductions in lateral root length and number than wild-type plants in response to high nitrate concentration. PMID: 19732351
  20. EIN2 membrane protein is required for both the first and second phases of growth inhibition by ethylene in Arabidopsis seedling hypocotyls. PMID: 15466219
  21. RCN1 and EIN2 modulate hypocotyl gravitropism and ethylene responses through independent pathways. PMID: 16798939
  22. EIN2 is a cross-link node in ethylene, ABA and stress signaling pathways, and EIN2 is necessary to induce developmental arrest during seed germination, and seedling establishment, as well as subsequent vegetative growth PMID: 17533512
  23. The results suggest that EIN2 gene mediates Pb(II) resistance, at least in part, through two distinct mechanisms, a GSH-dependent mechanism and a GSH-independent AtPDR12-mediated mechanism. PMID: 19153049
  24. Overexpression of ETP1 or ETP2 disrupts EIN2 protein accumulation, and these plants manifest a strong ethylene-insensitive phenotype PMID: 19196655
  25. ORE1 positively regulates aging-induced cell death; ORE1 expression is upregulated with leaf aging by EIN2 but negatively regulated by miR164; feed-forward pathway involving ORE1, miR164 & EIN2 provides regulation ensuring aging induces cell death PMID: 19229035
  26. These results suggest that ethylene signals may be transduced by a phosphor-relay from receptors to transcriptional events via both ein2-dependent and -independent pathways. PMID: 19253305

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Subcellular Location
[Ethylene-insensitive protein 2]: Endoplasmic reticulum membrane; Multi-pass membrane protein.; [EIN2-CEND]: Nucleus. Cytoplasm. Endoplasmic reticulum membrane.
Protein Families
NRAMP (TC 2.A.55) family
Tissue Specificity
[EIN2-CEND]: Localized to the guard cells after methyl jasmonate treatment.
Database Links

KEGG: ath:AT5G03280

STRING: 3702.AT5G03280.1

UniGene: At.1598

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