EIN3 Antibody

Code CSB-PA515845XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) EIN3 Polyclonal antibody
Uniprot No.
Target Names
EIN3
Alternative Names
EIN3 antibody; At3g20770 antibody; MOE17.8Protein ETHYLENE INSENSITIVE 3 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana EIN3 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Probable transcription factor acting as a positive regulator in the ethylene response pathway. Is required for ethylene responsiveness in adult plant tissues. Binds a primary ethylene response element present in the ETHYLENE-RESPONSE-FACTOR1 promoter with consequence to activate the transcription of this gene.
Gene References into Functions
  1. EIN3-SOS2 might link the ethylene signaling pathway and the SOS pathway in Arabidopsis salt responses. PMID: 28300216
  2. ET-activated transcription factor ETHYLENE-INSENSITIVE 3 (EIN3) physically interacts with ROOT HAIR DEFECTIVE 6 (RHD6), a well-documented positive regulator of hair cells, and that the two factors directly coactivate the hair length-determining gene RHD6-LIKE 4 (RSL4) to promote root hair elongation. PMID: 29233944
  3. the opposing function of two transcription factors, ethylene insensitive3 (EIN3) and EIN3-Like1 (EIL1), in salicylic acid-mediated suppression and jasmonic acid-mediated activation of PLANT DEFENSIN1.2 (PDF1.2), through ORA59. PMID: 28168848
  4. possible regulatory mechanism of ethylene in sulfur metabolism through direct EIN3-SLIM1 interaction PMID: 27968996
  5. EIN3, ORE1 and chlorophyll catabolic genes constitute a coherent feed-forward loop involving in the robust regulation of ethylene-mediated chlorophyll degradation during leaf senescence in Arabidopsis. PMID: 26218222
  6. High salinity induces EIN3 and EIL1 protein accumulation and activated EIN3 deters excess reactive oxygen species accumulation and increases salt tolerance. PMID: 25330213
  7. The signaling pathway involving EIN2-EIN3-miR164-NAC2 regulates leaf senescence and provides a mechanistic insight into how ethylene promotes the progression of leaf senescence in Arabidopsis thaliana. PMID: 24064769
  8. MYC2 physically interacts with EIN3 and inhibits its DNA binding activity PMID: 24668749
  9. MYC2 interacts with EIN3 to attenuate the transcriptional activity of EIN3 and repress ET-enhanced apical hook curvature. PMID: 24399301
  10. YID1/MED16, together with MED25, regulate iron homeostasis by interacting with EIN3/EIL1 and regulating the expression of downstream genes. PMID: 24456400
  11. Ethylene via EIN3 concomitantly activates two contrasting pathways: the PIF3-dependent growth-promoting pathway and an ethylene response factor 1 (ERF1)-mediated growth-inhibiting pathway. PMID: 22818915
  12. At moderate doses of salinity, partial insensitivity to ethylene might be compensated by changes in endogenous levels of other phytohormones and lipid peroxidation-derived signals in the ein3-1 mutant exposed to salt stress. PMID: 22209220
  13. the transcriptional complex of EIN3-ESE1 is a crucial event in the salt response, thereby connecting the transcriptional regulation of EIN3 and the downstream protein ESE1 in the salt response. PMID: 21832142
  14. Transcription factor EIN3 is required for the second phase but not the first phase of growth inhibition by ethylene in Arabidopsis seedling hypocotyls. PMID: 15466219
  15. Light induced regulation of gene expression involves stabilization of EIN3 protein. PMID: 17011517
  16. The bifurcate and antagonistic CTR1 and MKK9 pathways are both critical in determining ethylene-signalling specificity through two MAPK phosphorylation sites with opposite effects on EIN3 stability PMID: 18273012
  17. An ethylene signal elevates the levels of EIN3, which directly binds and activates the EBF2 gene promoter. PMID: 18466304
  18. A mutation in the EIN3 gene delayed the water-stress related increase in alpha-tocopherol and caused a reduction in the levels of this antioxidant by ca. 30% compared to the wild type. PMID: 19258016

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Subcellular Location
Nucleus.
Protein Families
EIN3 family
Database Links

KEGG: ath:AT3G20770

STRING: 3702.AT3G20770.1

UniGene: At.24824

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