Recombinant Arabidopsis thaliana Protein ETHYLENE INSENSITIVE 3 (EIN3), partial

Code CSB-YP515845DOA
MSDS
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Source Yeast
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Code CSB-EP515845DOA
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Source E.coli
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Code CSB-EP515845DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP515845DOA
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Source Baculovirus
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Code CSB-MP515845DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
EIN3
Uniprot No.
Alternative Names
EIN3; At3g20770; MOE17.8Protein ETHYLENE INSENSITIVE 3
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Probable transcription factor acting as a positive regulator in the ethylene response pathway. Is required for ethylene responsiveness in adult plant tissues. Binds a primary ethylene response element present in the ETHYLENE-RESPONSE-FACTOR1 promoter with consequence to activate the transcription of this gene.
Gene References into Functions
  1. EIN3-SOS2 might link the ethylene signaling pathway and the SOS pathway in Arabidopsis salt responses. PMID: 28300216
  2. ET-activated transcription factor ETHYLENE-INSENSITIVE 3 (EIN3) physically interacts with ROOT HAIR DEFECTIVE 6 (RHD6), a well-documented positive regulator of hair cells, and that the two factors directly coactivate the hair length-determining gene RHD6-LIKE 4 (RSL4) to promote root hair elongation. PMID: 29233944
  3. the opposing function of two transcription factors, ethylene insensitive3 (EIN3) and EIN3-Like1 (EIL1), in salicylic acid-mediated suppression and jasmonic acid-mediated activation of PLANT DEFENSIN1.2 (PDF1.2), through ORA59. PMID: 28168848
  4. possible regulatory mechanism of ethylene in sulfur metabolism through direct EIN3-SLIM1 interaction PMID: 27968996
  5. EIN3, ORE1 and chlorophyll catabolic genes constitute a coherent feed-forward loop involving in the robust regulation of ethylene-mediated chlorophyll degradation during leaf senescence in Arabidopsis. PMID: 26218222
  6. High salinity induces EIN3 and EIL1 protein accumulation and activated EIN3 deters excess reactive oxygen species accumulation and increases salt tolerance. PMID: 25330213
  7. The signaling pathway involving EIN2-EIN3-miR164-NAC2 regulates leaf senescence and provides a mechanistic insight into how ethylene promotes the progression of leaf senescence in Arabidopsis thaliana. PMID: 24064769
  8. MYC2 physically interacts with EIN3 and inhibits its DNA binding activity PMID: 24668749
  9. MYC2 interacts with EIN3 to attenuate the transcriptional activity of EIN3 and repress ET-enhanced apical hook curvature. PMID: 24399301
  10. YID1/MED16, together with MED25, regulate iron homeostasis by interacting with EIN3/EIL1 and regulating the expression of downstream genes. PMID: 24456400
  11. Ethylene via EIN3 concomitantly activates two contrasting pathways: the PIF3-dependent growth-promoting pathway and an ethylene response factor 1 (ERF1)-mediated growth-inhibiting pathway. PMID: 22818915
  12. At moderate doses of salinity, partial insensitivity to ethylene might be compensated by changes in endogenous levels of other phytohormones and lipid peroxidation-derived signals in the ein3-1 mutant exposed to salt stress. PMID: 22209220
  13. the transcriptional complex of EIN3-ESE1 is a crucial event in the salt response, thereby connecting the transcriptional regulation of EIN3 and the downstream protein ESE1 in the salt response. PMID: 21832142
  14. Transcription factor EIN3 is required for the second phase but not the first phase of growth inhibition by ethylene in Arabidopsis seedling hypocotyls. PMID: 15466219
  15. Light induced regulation of gene expression involves stabilization of EIN3 protein. PMID: 17011517
  16. The bifurcate and antagonistic CTR1 and MKK9 pathways are both critical in determining ethylene-signalling specificity through two MAPK phosphorylation sites with opposite effects on EIN3 stability PMID: 18273012
  17. An ethylene signal elevates the levels of EIN3, which directly binds and activates the EBF2 gene promoter. PMID: 18466304
  18. A mutation in the EIN3 gene delayed the water-stress related increase in alpha-tocopherol and caused a reduction in the levels of this antioxidant by ca. 30% compared to the wild type. PMID: 19258016

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Subcellular Location
Nucleus.
Protein Families
EIN3 family
Database Links

KEGG: ath:AT3G20770

STRING: 3702.AT3G20770.1

UniGene: At.24824

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