Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) envZ Polyclonal antibody

Uniprot No.

P0AEJ4

Target Names

envZ

Alternative Names

envZ; ompB; perA; tpo; b3404; JW3367; Sensor histidine kinase EnvZ; Osmolarity sensor protein EnvZ

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) envZ protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Member of the two-component regulatory system EnvZ/OmpR involved in osmoregulation (particularly of genes ompF and ompC) as well as other genes. EnvZ functions as a membrane-associated protein kinase that phosphorylates OmpR in response to environmental signals; at low osmolarity OmpR activates ompF transcription, while at high osmolarity it represses ompF and activates ompC transcription. Also dephosphorylates OmpR in the presence of ATP. The cytoplasmic dimerization domain (CDD) forms an osmosensitive core; increasing osmolarity stabilizes this segment (possibly by its contraction), enhancing the autophosphorylation rate and consequently, downstream phosphotransfer to OmpR and signaling. Autophosphorylation is greater when full-length EnvZ is reconstituted in a lipid environment, lipid-mediated allostery impacts the kinase function of EnvZ. Involved in acid stress response; this requires EnvZ but not OmpR phosphorylation, and suggests that EnvZ senses cytoplasmic acidic pH.

Gene References into Functions

study also reveals that polyP-dependent stalling has little effect on EnvZ levels. Hence, both polyP motifs in EnvZ are primarily involved in protein-protein interaction. PMID: 29953503
A library of single-Cys-containing EnvZ proteins was used to show that the major TM1-TM1' interface falls along a single surface consisting of residue positions 19, 23, 26, 30 and 34. Cys substitutions in the N- and C-terminal regions of TM1 change EnvZ signal output. Core residues in TM1 cause TM1 dimerisation and maintenance of steady-state signal output. Extracellular osmolarity does not rearrange TM1-TM1' interfaces. PMID: 27155567
Cytoplasmic sensing by the inner membrane histidine kinase EnvZ PMID: 25937465
structure shows an asymmetric histidine kinase dimer where one subunit is caught performing the autophosphorylation reaction PMID: 24500224
Both the E. coli MG1655 and E. coli Nissle 1917 strains containing envZP41L produced more phosphorylated OmpR than their parents. PMID: 24478082
HDXMS data showed that the cytoplasmic domain of EnvZ exists as an ensemble of multiple conformations and osmolytes favoured increased helicity. High osmolality increased the autophosphorylation of His243. PMID: 22543870
This study report that 10 to 20% of motile E. coli MG1655 are envZ missense mutants that are better colonizers of the mouse intestine than E. coli MG1655. PMID: 22392928
Direct binding studies showed that the cytoplasmic domains of these proteins each form specific homodimers in vitro. PMID: 21854787
Together, the data suggest that the binding of MzrA to EnvZ influences the ability of EnvZ to receive and/or respond to environmental signals in the periplasm and modulate its biochemical output to OmpR. PMID: 20889743
Data show that PerA is required for activation of the operon LEE1, but that this activation is via autoactivation of the per promoter which ensures sufficient production of the PerC protein to activate LEE1. PMID: 15522091

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Subcellular Location

Cell inner membrane; Multi-pass membrane protein.

Database Links

KEGG: ecj:JW3367

STRING: 316385.ECDH10B_3579

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envZ Antibody

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