Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FAB1 Polyclonal antibody

Uniprot No.

P34756

Target Names

FAB1

Alternative Names

FAB1 antibody; YFR019W1-phosphatidylinositol 3-phosphate 5-kinase FAB1 antibody; Phosphatidylinositol 3-phosphate 5-kinase antibody; EC 2.7.1.150 antibody; Type III PIP kinase antibody; PIPkin-III antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FAB1 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Catalyzes the phosphorylation of phosphatidylinositol 3-phosphate on the fifth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 3,5-bisphosphate. Required for endocytic-vacuolar pathway and nuclear migration. The product of the reaction, PI(3,5)P2 is an important regulator of vacuole homeostasis perhaps by controlling membrane flux to and/or from the vacuole. PI(3,5)P2 regulates the transition between trans-SNARE complex formation and vacuole membrane fusion. Hyperosmotic shock-induced increase in the levels of PtdIns(3,5)P2 requires the presence of VAC7, VAC14, and/or FIG4.

Gene References into Functions

data suggest that Ivy1 modulates Fab1-mediated PI-3,5-P2 synthesis during membrane stress and may allow adjustment of the vacuole membrane environment. PMID: 29674454
Pho85/CDK5 provides protection against hyperosmotic stress and acts before long-term adaptation provided by Hog1. This protection requires the vacuolar/endolysosomal signaling lipid PI3,5P2 We show that Pho85/CDK5 directly phosphorylates and positively regulates the PI3P-5 kinase Fab1/PIKfyve complex and provide evidence that this regulation is conserved in mammalian cells. PMID: 28637746
ATG18 and FAB1 are involved in dehydration stress tolerance in Saccharomyces cerevisiae. PMID: 25803831
Data suggest a model in which PtdIns(3)P 5-kinase protein Fab1/PIKfyve is dispensable to maintain vacuolar/lysosomal acidification. PMID: 25713145
The crystal structure now unravels the presence of two distinct phosphoinositide-binding sites at the circumference of the seven bladed beta-propeller showing PtdINS3P and PtdIns(3,5)P2 proteins PMID: 23069643
Vac14 protein multimerization is a prerequisite step for Fab1 protein complex assembly and funct PMID: 23389034
Results show that Fab1p is required for AP-1-dependent process for trafficking of endogenously ubiquitylated cargoes to the vacuole lumen in S. cerevisiae. PMID: 17003107
Atg18p is likely a PtdIns(3,5)P(2)"sensor," acting as an effector to remodel membranes as well as regulating its synthesis via feedback that might involve Vac7p PMID: 17699591
Fab1 phosphoinositide kinase signaling complex assembly requires the Fig4 phosphoinositide phosphatase PMID: 18653468

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Subcellular Location

Vacuole membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein.

Database Links

KEGG: sce:YFR019W

STRING: 4932.YFR019W

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FAB1 Antibody

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