Recombinant Saccharomyces cerevisiae 1-phosphatidylinositol 3-phosphate 5-kinase FAB1 (FAB1), partial

Code CSB-YP327363SVG
MSDS
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Source Yeast
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Code CSB-EP327363SVG
MSDS
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Source E.coli
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Code CSB-EP327363SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP327363SVG
MSDS
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Source Baculovirus
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Code CSB-MP327363SVG
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
FAB1
Uniprot No.
Alternative Names
FAB1; YFR019W1-phosphatidylinositol 3-phosphate 5-kinase FAB1; Phosphatidylinositol 3-phosphate 5-kinase; EC 2.7.1.150; Type III PIP kinase; PIPkin-III
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Catalyzes the phosphorylation of phosphatidylinositol 3-phosphate on the fifth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 3,5-bisphosphate. Required for endocytic-vacuolar pathway and nuclear migration. The product of the reaction, PI(3,5)P2 is an important regulator of vacuole homeostasis perhaps by controlling membrane flux to and/or from the vacuole. PI(3,5)P2 regulates the transition between trans-SNARE complex formation and vacuole membrane fusion. Hyperosmotic shock-induced increase in the levels of PtdIns(3,5)P2 requires the presence of VAC7, VAC14, and/or FIG4.
Gene References into Functions
  1. data suggest that Ivy1 modulates Fab1-mediated PI-3,5-P2 synthesis during membrane stress and may allow adjustment of the vacuole membrane environment. PMID: 29674454
  2. Pho85/CDK5 provides protection against hyperosmotic stress and acts before long-term adaptation provided by Hog1. This protection requires the vacuolar/endolysosomal signaling lipid PI3,5P2 We show that Pho85/CDK5 directly phosphorylates and positively regulates the PI3P-5 kinase Fab1/PIKfyve complex and provide evidence that this regulation is conserved in mammalian cells. PMID: 28637746
  3. ATG18 and FAB1 are involved in dehydration stress tolerance in Saccharomyces cerevisiae. PMID: 25803831
  4. Data suggest a model in which PtdIns(3)P 5-kinase protein Fab1/PIKfyve is dispensable to maintain vacuolar/lysosomal acidification. PMID: 25713145
  5. The crystal structure now unravels the presence of two distinct phosphoinositide-binding sites at the circumference of the seven bladed beta-propeller showing PtdINS3P and PtdIns(3,5)P2 proteins PMID: 23069643
  6. Vac14 protein multimerization is a prerequisite step for Fab1 protein complex assembly and funct PMID: 23389034
  7. Results show that Fab1p is required for AP-1-dependent process for trafficking of endogenously ubiquitylated cargoes to the vacuole lumen in S. cerevisiae. PMID: 17003107
  8. Atg18p is likely a PtdIns(3,5)P(2)"sensor," acting as an effector to remodel membranes as well as regulating its synthesis via feedback that might involve Vac7p PMID: 17699591
  9. Fab1 phosphoinositide kinase signaling complex assembly requires the Fig4 phosphoinositide phosphatase PMID: 18653468

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Subcellular Location
Vacuole membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein.
Database Links

KEGG: sce:YFR019W

STRING: 4932.YFR019W

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