FAD2 Antibody

Code CSB-PA342791XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) FAD2 Polyclonal antibody
Uniprot No.
Target Names
FAD2
Alternative Names
FAD2; At3g12120; T21B14.6; T21B14_107; T23B7.6; Delta(12-fatty-acid desaturase; Fatty acid desaturase 2; Omega-6 fatty acid desaturase, endoplasmic reticulum
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana FAD2 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
ER (microsomal) omega-6 fatty acid desaturase introduces the second double bond in the biosynthesis of 18:3 fatty acids, important constituents of plant membranes. Delta(12)-desaturase with regioselectivity determined by the double bond (delta(9) position) and carboxyl group of the substrate. Can use both 16:1 and 18:1 fatty acids as substrates. It is thought to use cytochrome b5 as an electron donor and to act on fatty acids esterified to phosphatidylcholine (PC) and, possibly, other phospholipids. Very low constitutive hydroxylation activity. Required for desaturation of fatty acids present in extraplastidial membranes, including mitochondria. Required for salt tolerance during seed germination and early seedling growth.
Gene References into Functions
  1. Data suggest that omega-6 desaturation is largely controlled by cis-acting sequence variants in the fatty acid desaturase 2 (FAD2) 5' untranslated region intron that determine the expression level of the gene. PMID: 28108698
  2. FAD2 and FATB may have influenced the adaptive evolution of seed oil composition to optimize seed oil melting point relative to germination temperatures PMID: 26865732
  3. Results suggest that FAD2 mediated high-level vacuolar and plasma membrane fatty acid desaturation is essential for the proper function of membrane attached Na+/H+ exchangers. PMID: 22279586
Subcellular Location
Endoplasmic reticulum membrane; Multi-pass membrane protein. Microsome membrane; Multi-pass membrane protein.
Protein Families
Fatty acid desaturase type 1 family
Tissue Specificity
Expressed in shoots and roots. Expressed in leaves, stems, flowers and siliques.
Database Links

KEGG: ath:AT3G12120

STRING: 3702.AT3G12120.1

UniGene: At.23898

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