Recombinant Arabidopsis thaliana Omega-6 fatty acid desaturase, endoplasmic reticulum (FAD2)

Code CSB-CF342791DOA
MSDS
Size Pls inquire
Source in vitro E.coli expression system
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Product Details

Target Names
FAD2
Uniprot No.
Alternative Names
FAD2; At3g12120; T21B14.6; T21B14_107; T23B7.6; Delta(12-fatty-acid desaturase; Fatty acid desaturase 2; Omega-6 fatty acid desaturase, endoplasmic reticulum
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-383
Target Protein Sequence
MGAGGRMPVPTSSKKSETDTTKRVPCEKPPFSVGDLKKAIPPHCFKRSIPRSFSYLISDI IIASCFYYVATNYFSLLPQPLSYLAWPLYWACQGCVLTGIWVIAHECGHHAFSDYQWLDD TVGLIFHSFLLVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYGKYLNNPLGRIM MLTVQFVLGWPLYLAFNVSGRPYDGFACHFFPNAPIYNDRERLQIYLSDAGILAVCFGLY RYAAAQGMASMICLYGVPLLIVNAFLVLITYLQHTHPSLPHYDSSEWDWLRGALATVDRD YGILNKVFHNITDTHVAHHLFSTMPHYNAMEATKAIKPILGDYYQFDGTPWYVAMYREAK ECIYVEPDREGDKKGVYWYNNKL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
ER (microsomal) omega-6 fatty acid desaturase introduces the second double bond in the biosynthesis of 18:3 fatty acids, important constituents of plant membranes. Delta(12)-desaturase with regioselectivity determined by the double bond (delta(9) position) and carboxyl group of the substrate. Can use both 16:1 and 18:1 fatty acids as substrates. It is thought to use cytochrome b5 as an electron donor and to act on fatty acids esterified to phosphatidylcholine (PC) and, possibly, other phospholipids. Very low constitutive hydroxylation activity. Required for desaturation of fatty acids present in extraplastidial membranes, including mitochondria. Required for salt tolerance during seed germination and early seedling growth.
Gene References into Functions
  1. Data suggest that omega-6 desaturation is largely controlled by cis-acting sequence variants in the fatty acid desaturase 2 (FAD2) 5' untranslated region intron that determine the expression level of the gene. PMID: 28108698
  2. FAD2 and FATB may have influenced the adaptive evolution of seed oil composition to optimize seed oil melting point relative to germination temperatures PMID: 26865732
  3. Results suggest that FAD2 mediated high-level vacuolar and plasma membrane fatty acid desaturation is essential for the proper function of membrane attached Na+/H+ exchangers. PMID: 22279586
Subcellular Location
Endoplasmic reticulum membrane; Multi-pass membrane protein. Microsome membrane; Multi-pass membrane protein.
Protein Families
Fatty acid desaturase type 1 family
Tissue Specificity
Expressed in shoots and roots. Expressed in leaves, stems, flowers and siliques.
Database Links

KEGG: ath:AT3G12120

STRING: 3702.AT3G12120.1

UniGene: At.23898

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