fepA Antibody

Datasheet

Code	CSB-PA361633XA01ENV
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Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) fepA Polyclonal antibody

Uniprot No.

P05825

Target Names

fepA

Alternative Names

fepA antibody; fep antibody; feuB antibody; b0584 antibody; JW5086Ferrienterobactin receptor antibody; Enterobactin outer-membrane receptor antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) fepA protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

This protein is involved in the initial step of iron uptake by binding ferrienterobactin (Fe-ENT), an iron chelatin siderophore that allows E.coli to extract iron from the environment. FepA also acts as a receptor for colicins B and D.

Gene References into Functions

A stem-loop within E. coli fepA mRNA coding region activates translation initiation. PMID: 28918899
TonB and FepA display confined mobility within Escherichia coli cell and inner membranes. PMID: 27935943
The authors found numerous specific sites of FepA interaction with TonB on the periplasmic face of the FepA cork in addition to the TonB box. PMID: 28264993
When FepA binds to ferric enterobactin in outer bacterial membrane, it was reflected by conformational motion of the external vestibular loops. PMID: 24981231
analysis of the uptake of ferric enterobactin (FeEnt) by Escherichia coli FepA PMID: 20335169
analysis of the mechanism of transport of ferric enterobactin through FepA PMID: 17056600
TonB was required for colicin-induced exposure of the FepA TonB box, suggesting that TonB binds FepA at a different site prior to interaction with the TonB box. PMID: 17578453
Modification of FepA by fluorescein maleimide was temperature-dependent, and was inhibited by colicin B binding to FepA. PMID: 19432807

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Subcellular Location

Cell outer membrane; Multi-pass membrane protein.

Protein Families

TonB-dependent receptor family

Database Links

KEGG: ecj:JW5086

STRING: 316385.ECDH10B_0651

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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fepA Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background