fepA Antibody

Datasheet

Code	CSB-PA361633XA11ENV
Size	US$299
Image	Western Blot Positive WB detected in: CSB-PA361633XA11ENV(40ng), CSB-PA361633XA11ENV(20ng),CSB-PA361633XA11ENV(10ng),CSB-PA361633XA11ENV(5ng) All lanes: fepA antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 87 kDa Observed band size: 100 kDa Exposure time: 120s
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Target Background

Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) fepA Polyclonal antibody

Uniprot No.

P05825

Target Names

fepA

Alternative Names

Ferrienterobactin receptor (Enterobactin outer-membrane receptor) fepA fep feuB b0584 JW5086

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) fepA protein (23-746aa )

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

This protein is involved in the initial step of iron uptake by binding ferrienterobactin (Fe-ENT), an iron chelatin siderophore that allows E.coli to extract iron from the environment. FepA also acts as a receptor for colicins B and D.

Gene References into Functions

A stem-loop within E. coli fepA mRNA coding region activates translation initiation. PMID: 28918899
TonB and FepA display confined mobility within Escherichia coli cell and inner membranes. PMID: 27935943
The authors found numerous specific sites of FepA interaction with TonB on the periplasmic face of the FepA cork in addition to the TonB box. PMID: 28264993
When FepA binds to ferric enterobactin in outer bacterial membrane, it was reflected by conformational motion of the external vestibular loops. PMID: 24981231
analysis of the uptake of ferric enterobactin (FeEnt) by Escherichia coli FepA PMID: 20335169
analysis of the mechanism of transport of ferric enterobactin through FepA PMID: 17056600
TonB was required for colicin-induced exposure of the FepA TonB box, suggesting that TonB binds FepA at a different site prior to interaction with the TonB box. PMID: 17578453
Modification of FepA by fluorescein maleimide was temperature-dependent, and was inhibited by colicin B binding to FepA. PMID: 19432807

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Subcellular Location

Cell outer membrane; Multi-pass membrane protein.

Protein Families

TonB-dependent receptor family

Database Links

KEGG: ecj:JW5086

STRING: 316385.ECDH10B_0651

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Protein Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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fepA Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background