FHY3 Antibody

Code CSB-PA867944XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) FHY3 Polyclonal antibody
Uniprot No.
Target Names
FHY3
Alternative Names
FHY3 antibody; At3g22170 antibody; MKA23.12Protein FAR-RED ELONGATED HYPOCOTYL 3 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana FHY3 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Transcription activator that recognizes and binds to the DNA consensus sequence 5'-CACGCGC-3'. Activates the expression of FHY1 and FHL involved in light responses. When associated with PHYA, protects it from being recognized and degraded by the COP1/SPA complex. Positive regulator of chlorophyll biosynthesis via the activation of HEMB1 gene expression.
Gene References into Functions
  1. the light-signaling proteins FHY3 and FAR1 influence starch synthesis and starch granule formation through transcriptional activation of ISA2. PMID: 27859295
  2. FHY3 and FAR1 directly bind the promoter of MIPS1 to activate its expression and thereby promote inositol biosynthesis to prevent PMID: 26714049
  3. this study demonstrates an important role of FHY3 and FAR1 in regulating plant immunity, through integrating chlorophyll biosynthesis and the salicylic acid signaling pathway. PMID: 25989254
  4. domestication of FHY3/FAR1 may enable angiosperms to better integrate various endogenous and exogenous signals for coordinated regulation of growth and development, thus enhancing their fitness and adaptation PMID: 25956482
  5. The fhy3 phenotypes of axillary bud outgrowth suppression and of stress-induced leaf growth retardation both required the AUXIN-RESISTANT1 gene, and are independent of phyA. PMID: 22540368
  6. FHY3 and FAR1 are positive regulators of abscissic acid (ABA) signaling and provide insight into the integration of light and ABA signaling. PMID: 23946351
  7. FRS4/CPD25 and FHY3/CPD45 function as a heterodimer that cooperatively activates ARC5. PMID: 23662592
  8. Data suggest that FHY3 regulates multiple facets of plant development, thus providing insights into its functions beyond light and circadian pathways. PMID: 21803941
  9. FHY3 appears to be a novel gating factor, specifically in gating red light signaling to the clock during daytime PMID: 17012604
  10. study shows FHY3 & FAR1, which encode proteins related to Mutator-like transposases, act together to modulate phyA signaling by activating transcription of FHY1 & FHL PMID: 18033885
  11. The zinc finger domain is essential for direct DNA binding of FHY3 in mediating light signaling. The central core transposase domain and C-terminal SWIM domain are essential for the transcriptional regulatory activity of FHY3. PMID: 18715961

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Subcellular Location
Nucleus.
Protein Families
FHY3/FAR1 family
Database Links

KEGG: ath:AT3G22170

STRING: 3702.AT3G22170.1

UniGene: At.27725

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