Recombinant Arabidopsis thaliana Protein FAR-RED ELONGATED HYPOCOTYL 3 (FHY3), partial

Code CSB-YP867944DOA
MSDS
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Source Yeast
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Code CSB-EP867944DOA
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Source E.coli
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Code CSB-EP867944DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP867944DOA
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Source Baculovirus
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Code CSB-MP867944DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
FHY3
Uniprot No.
Alternative Names
FHY3; At3g22170; MKA23.12Protein FAR-RED ELONGATED HYPOCOTYL 3
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcription activator that recognizes and binds to the DNA consensus sequence 5'-CACGCGC-3'. Activates the expression of FHY1 and FHL involved in light responses. When associated with PHYA, protects it from being recognized and degraded by the COP1/SPA complex. Positive regulator of chlorophyll biosynthesis via the activation of HEMB1 gene expression.
Gene References into Functions
  1. the light-signaling proteins FHY3 and FAR1 influence starch synthesis and starch granule formation through transcriptional activation of ISA2. PMID: 27859295
  2. FHY3 and FAR1 directly bind the promoter of MIPS1 to activate its expression and thereby promote inositol biosynthesis to prevent PMID: 26714049
  3. this study demonstrates an important role of FHY3 and FAR1 in regulating plant immunity, through integrating chlorophyll biosynthesis and the salicylic acid signaling pathway. PMID: 25989254
  4. domestication of FHY3/FAR1 may enable angiosperms to better integrate various endogenous and exogenous signals for coordinated regulation of growth and development, thus enhancing their fitness and adaptation PMID: 25956482
  5. The fhy3 phenotypes of axillary bud outgrowth suppression and of stress-induced leaf growth retardation both required the AUXIN-RESISTANT1 gene, and are independent of phyA. PMID: 22540368
  6. FHY3 and FAR1 are positive regulators of abscissic acid (ABA) signaling and provide insight into the integration of light and ABA signaling. PMID: 23946351
  7. FRS4/CPD25 and FHY3/CPD45 function as a heterodimer that cooperatively activates ARC5. PMID: 23662592
  8. Data suggest that FHY3 regulates multiple facets of plant development, thus providing insights into its functions beyond light and circadian pathways. PMID: 21803941
  9. FHY3 appears to be a novel gating factor, specifically in gating red light signaling to the clock during daytime PMID: 17012604
  10. study shows FHY3 & FAR1, which encode proteins related to Mutator-like transposases, act together to modulate phyA signaling by activating transcription of FHY1 & FHL PMID: 18033885
  11. The zinc finger domain is essential for direct DNA binding of FHY3 in mediating light signaling. The central core transposase domain and C-terminal SWIM domain are essential for the transcriptional regulatory activity of FHY3. PMID: 18715961

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Subcellular Location
Nucleus.
Protein Families
FHY3/FAR1 family
Database Links

KEGG: ath:AT3G22170

STRING: 3702.AT3G22170.1

UniGene: At.27725

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