FIE Antibody

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Code	CSB-PA862881XA01DOA
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Target Background

Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) FIE Polyclonal antibody

Uniprot No.

Q9LT47

Target Names

FIE

Alternative Names

FIE antibody; FIS3 antibody; At3g20740 antibody; MOE17.5 antibody; Polycomb group protein FERTILIZATION-INDEPENDENT ENDOSPERM antibody; Protein FERTILIZATION-INDEPENDENT SEED 3 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana FIE protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

Polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. They probably act via the methylation of histones, rendering chromatin heritably changed in its expressibility. Required to prevent the proliferation of the central cell by repressing unknown target genes before fertilization. Probably also involved in floral repression mechanism established during early plant development. Regulates the anteroposterior organization of the endosperm. Interacts with the promoter and represses the transcription of genes such as PHE1, that are paternally active and maternally silenced.

Gene References into Functions

Arabidopsis FIE binding sites tend to be in gene coding regions and co-localize with H3K27me3. PMID: 24001316
H3K27me3 deposition is abolished genome-wide in fertilization independent endosperm protein mutants demonstrating the essential function of polycomb repressive complex 2 PMID: 21423668
Used to demonstrate the feasibility of bimolecular fluorescence complementation in plants. PMID: 15469499

Subcellular Location

Nucleus. Note=Excluded from the nucleolus.

Protein Families

WD repeat ESC family

Tissue Specificity

Expressed in cauline leaves, root and stems. In the male reproductive organ, it is expressed in the developing anther; and is abundant in microspore mother cells, in microsporocytes and in the tapetum, but is absent from vascular bundles, the connective t

Database Links

KEGG: ath:AT3G20740

STRING: 3702.AT3G20740.1

UniGene: At.20884

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FIE Antibody

Product Details

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Target Background