Recombinant Arabidopsis thaliana Polycomb group protein FERTILIZATION-INDEPENDENT ENDOSPERM (FIE)

Code CSB-YP862881DOA
MSDS
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Source Yeast
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Code CSB-EP862881DOA
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Source E.coli
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Code CSB-EP862881DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP862881DOA
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Source Baculovirus
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Code CSB-MP862881DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
FIE
Uniprot No.
Alternative Names
FIE; FIS3; At3g20740; MOE17.5; Polycomb group protein FERTILIZATION-INDEPENDENT ENDOSPERM; Protein FERTILIZATION-INDEPENDENT SEED 3
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-369
Target Protein Sequence
MSKITLGNES IVGSLTPSNK KSYKVTNRIQ EGKKPLYAVV FNFLDARFFD VFVTAGGNRI TLYNCLGDGA ISALQSYADE DKEESFYTVS WACGVNGNPY VAAGGVKGII RVIDVNSETI HKSLVGHGDS VNEIRTQPLK PQLVITASKD ESVRLWNVET GICILIFAGA GGHRYEVLSV DFHPSDIYRF ASCGMDTTIK IWSMKEFWTY VEKSFTWTDD PSKFPTKFVQ FPVFTASIHT NYVDCNRWFG DFILSKSVDN EILLWEPQLK ENSPGEGASD VLLRYPVPMC DIWFIKFSCD LHLSSVAIGN QEGKVYVWDL KSCPPVLITK LSHNQSKSVI RQTAMSVDGS TILACCEDGT IWRWDVITK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. They probably act via the methylation of histones, rendering chromatin heritably changed in its expressibility. Required to prevent the proliferation of the central cell by repressing unknown target genes before fertilization. Probably also involved in floral repression mechanism established during early plant development. Regulates the anteroposterior organization of the endosperm. Interacts with the promoter and represses the transcription of genes such as PHE1, that are paternally active and maternally silenced.
Gene References into Functions
  1. Arabidopsis FIE binding sites tend to be in gene coding regions and co-localize with H3K27me3. PMID: 24001316
  2. H3K27me3 deposition is abolished genome-wide in fertilization independent endosperm protein mutants demonstrating the essential function of polycomb repressive complex 2 PMID: 21423668
  3. Used to demonstrate the feasibility of bimolecular fluorescence complementation in plants. PMID: 15469499
Subcellular Location
Nucleus. Note=Excluded from the nucleolus.
Protein Families
WD repeat ESC family
Tissue Specificity
Expressed in cauline leaves, root and stems. In the male reproductive organ, it is expressed in the developing anther; and is abundant in microspore mother cells, in microsporocytes and in the tapetum, but is absent from vascular bundles, the connective t
Database Links

KEGG: ath:AT3G20740

STRING: 3702.AT3G20740.1

UniGene: At.20884

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